Solid phase extraction columnIt is a laboratory consumable used for sample pretreatment, which separates and enriches target compounds from complex matrices through selective adsorption of adsorbents. It is widely used in analysis and detection in fields such as food, environment, and medicine. Its core function is to improve the accuracy and sensitivity of detection. Common adsorbents include HLB, C18, etc., which are suitable for compounds of different polarities.
Application Scenario:
Environmental monitoring: such as pesticide and polychlorinated biphenyls (PCBs) testing.
Food testing: such as analysis of additives and residues.
Drug analysis: such as drug enrichment in blood and urine.
The correct use of solid-phase extraction columns:
1. Activation
Slowly pass 3-5 mL of organic solvent (such as methanol) through the column, wet the packing, and remove impurities. Pay attention to controlling the flow rate at 1-2mL/min to avoid insufficient activation caused by too fast a flow rate.
2. Balance
Replace the activating solvent with a solvent with similar properties to the sample, such as water or buffer, to adapt the filler to the loading environment and ensure effective binding of the target substance.
3. Sample loading
Slowly add the preprocessed sample (5-20mL) into the column, with a flow rate controlled at 1-3mL/min. Turbid samples need to slow down the flow rate to avoid loss of target material.
4. Rinsing
Rinse impurities with a weak solvent (such as 5% methanol aqueous solution) at a flow rate of 1-2 mL/min. The rinse solution should be able to remove interfering substances but retain the target substance.
5. Elution
Wash the target substance with a strong solvent (such as pure acetonitrile) at a flow rate of 0.5-1 mL/min. The elution can be repeated once to improve the recovery rate.