The gel permeation chromatographic column conforming to the determination method of organochlorine pesticide residues in the 2015 edition of the Chinese Pharmacopoeia has been upgraded!
1： Adjusting the sand core model makes the flow rate easier to control, as required by the pharmacopoeia.
2： Add standard grinding mouth and standard plug to prevent solvent volatilization, facilitate sealing and storage, and extend the service life of gel.
3： Add pressure devices to improve experimental efficiency.
Upgrade once to meet your comprehensive needs. What changes are quality and performance, while what remains unchanged are price and service. Old users can enjoy lower price upgrades!
In addition, our company provides technical guidance for Bio beads S-X3 polystyrene gel and column installation. Welcome new and old customers to consult!

2015Method for determination of pesticide residues in the Chinese Pharmacopoeia

Preparation of test solution:Take the test sample,Grind into powder(Passing the third sieve),Take an appointment1.5g,weigh accurately,placed in50mlPolystyrene plug centrifuge tube,Intense shaking extraction1minute,Add pre weighed anhydrous magnesium sulfate again4gCompared to sodium chloride5gThe mixed powder,Shake vigorously again1Minutes later,centrifugation(4000turn/divide)1minute. Precision suction of supernatant10ml,40℃Concentrate under reduced pressure to near dryness,Using cyclohexane ethyl acetate(-1∶1)Transfer the mixed solution in batches to10mlIn the measuring bottle,Add cyclohexane-Ethyl acetate（1∶1)Mix the solution to the mark,Shake well,Transfer to pre join1gIn the centrifuge tube of anhydrous sodium sulfate,shake,place1hour,centrifugation(Filter if necessary)Take the supernatant5ml,Through gel permeation chromatographic column(400mm×25mm,contentsBIO-Beads S-X3filler;Using cyclohexane-ethyl acetate(1∶1)Mixed solution as mobile phase;Flow rate is per minute5.0ml)purify,collect18~30Minute elution solution, in40℃ water bathConcentrate under reduced pressure to near dryness, replace twice with a small amount of n-hexane, and add n-hexane1mlDissolve and transfer to the Florisil solid-phase extraction column[1000mg/6ml,Using n-hexane-Acetone（95:5）Mixed solution10mlAnd n-hexane10mlPre wash]Wash the residue with n-hexane3Every time, every time1ml,Transfer the washing solution to the same Florisil solid-phase extraction column, and then use n-hexane-Acetone（95:5）Mixed solution10mlElute, collect all eluent, place it on a nitrogen blower until almost dry, add isooctane to make up to volume1ml,Vortex dissolves, that's it.

2015Chinese Pharmacopoeia Edition Sample preparation and purification required for pesticide residue determination method:

No.AA400253Gel permeation chromatographic column (empty column)400mm*25mm

152-2750 BIO-Beads S-X3filler200-400The eyes,100g/bottle

HSC-12B 12Circular water bath nitrogen blower

57057 Florisil solid phase extraction column1000mg/6ml,30branch/box