1. A dedicated person is responsible for use: avoid arbitrarily twisting the switches and adjusting the knobs on the control panel to prevent affecting the stability of temperature, carbon dioxide concentration, and humidity inside the box, while reducing the sensitivity of the machine.

2. Proper operation of temperature sensor: The temperature sensor requires a room temperature of 25 ° C for normal operation. When the room temperature exceeds 30 ° C, the adjustment function of the temperature sensor may fail and the room temperature needs to be lowered to restore normal operation. During summer use, the sterile room should maintain a room temperature of approximately 25 ° C.

3. Gas sensitive protection: Control the pressure changes inside the control box, and the intake pressure should be 0.06 megapascals. Avoid rapid inflation with excessive pressure to prevent damage to the CO ₂ sensor. It is recommended to use the method of "counterclockwise loosening, gas bottle connection, clockwise pressure reduction, pressure 0.06" to adjust the pressure and conduct an air tightness test.

4. Monitor CO ₂ zero drift: Use a CO ₂ gas detector to regularly check the gas. The laboratory should establish a CO ₂ pressure zero point testing system, which is generally tested every 3 months to ensure that the actual usage value of CO ₂ pressure is correct.

5. Humidity monitoring of incubator: Place a certain amount of sterilizing water on the incubator tray to keep the incubator in a humidity saturated environment, in order to meet the requirements of open cell culture.

6. Other precautions: Control different systems according to the purpose of use, such as turning off the carbon dioxide control system during high-precision constant temperature cultivation; When an over temperature alarm occurs, the switch should be turned off and wait for 30 minutes before turning it back on; When cleaning the studio, use professional tools to avoid collision with sensors and mixing motor rotors; Remove the shelf and humidification tray before transportation to avoid inverting the instrument