Low oxygen incubator is an experimental equipment used to simulate low oxygen environments, widely used in cell culture, microbial culture, and biological research. By accurately controlling the oxygen concentration inside the box, a simulated low oxygen or hypoxic environment is provided to help researchers study the effects of low oxygen conditions on cells, organisms, diseases, and other aspects.
The working principle of a low oxygen incubator mainly includes the following aspects:
Oxygen concentration control: The built-in oxygen sensor monitors the oxygen concentration inside the box to ensure that it remains within the set range. The reduction of oxygen concentration is usually achieved by introducing nitrogen into the incubator. Nitrogen, as an inert gas, does not participate in biological reactions and can effectively reduce oxygen concentration.
Carbon dioxide concentration control: In order to ensure the acid-base balance of the cultivation environment, low oxygen incubators are usually equipped with carbon dioxide control systems. Carbon dioxide plays an important role in cell culture, helping to maintain the pH stability of the culture medium.
Temperature control: Temperature is a key factor affecting cell growth and metabolism. A low oxygen incubator needs to maintain a certain temperature (usually around 37 ℃) to simulate the physiological environment inside the organism.
Gas circulation system: In order to ensure uniform distribution of low oxygen environment, a gas circulation system is usually equipped. The system uses fans or airflow devices to evenly distribute the gas inside the box, preventing uneven oxygen concentration in different areas.
Airtight design: With high sealing performance, it can effectively prevent the infiltration of external air, thereby maintaining a stable oxygen concentration inside the box.
Precautions for use
Environmental control: Regularly calibrate sensors, use high-purity gas, and avoid frequent opening and closing of box doors.
Safe operation: It is strictly prohibited to place flammable materials in the box, and flammable sprays shall not be used nearby.
Maintenance: Regularly clean the interior, gas channels, and sensors to ensure equipment performance.
Installation requirements: Place in an environment with less dust and minimal temperature changes, ground the box and use a three pin plug.