In synthetic biology experiments, monitoring the process is crucial for efficient cultivation and productivity improvement. For the issue of electrode sterilization, it can be solved by batch sterilization and one-time calibration, which is convenient to operate. In the experiment, the large-scale laboratory is equipped with multiple bioreactors, which can be synchronously cultured under the same strain and different process conditions. Real time monitoring of pH, dissolved oxygen, temperature and other sensors is used to provide a suitable growth environment for microorganisms. At the same time, experimental data is converted into production data, greatly reducing process exploration time and improving production capacity.
The monitoring data can clearly present key information such as pH value, dissolved oxygen value, temperature, cultivation duration, tank consistency, and strain yield under different processes, and the data consistency is high. Taking a laboratory case as an example, it used more than ten stainless steel reactors to cultivate Escherichia coli and produce biological raw materials. The detection system was provided by the speaker, the overall process system was provided by the Beijing company, and the process package was provided by the Shanghai company. Multiple parties cooperated to ensure the smooth progress of the experiment.
These biological cultivation technologies have been widely applied in emerging industries: Kaisai Biotechnology uses genetically modified Escherichia coli to produce new materials from straw; Biodegradable biomaterials can replace synthetic plastics in petrochemicals and be applied in fields such as plastic film, making it more environmentally friendly and efficient; Feed, nutritional supplements, insulin, etc. are also mostly produced through biological methods.
Synthetic biology, as a multidisciplinary field, promotes the application of microbial technology in new materials, environmental protection, medicine and other fields through precise monitoring and process optimization. It is expected to profoundly change the development of the industry and people's lives in the future.