The core tool of synthetic biology high-temperature pharmaceuticals isbioreactorIts essence is to screen suitable bacterial strains through multiple rounds and batches of experimentsexcellentThe technology system for cultivating the environment is widely used in the laboratories of companies such as Kaisai Biotechnology and Huaxi Biotechnology, supporting the research and production of pharmaceuticals and biological products.
Bioreactors are divided into two categories based on their scale: commonly used in laboratoriesSmall reactor（500ml、1L、2L、3L、5L）， Multi row layout can conduct multiple experiments simultaneously; After successful screening, transfer toLarge scale reactorTo achieve increased production and capacity. All reactors must be equipped with three types of key equipment: firstlyPH sensorAdjust the pH value of the environment to create suitable growth conditions for microorganisms; SecondDissolved oxygen sensorMonitor ventilation and oxygen levels to ensure microbial respiratory metabolism, especially when oxygen levels decrease after adding sugar, which can provide real-time warning; three isTriE Conductivity SensorTest the conductivity of raw water to ensure that the basic water quality meets the standard.
Microbial cultivation requires nutrients such as carbon sources (such as glucose) and nitrogen sources (animal/plant nitrogen sources). Adding sugar can lead to a decrease in oxygen content, and ventilation needs to be dynamically adjusted through dissolved oxygen sensors. The experimental process follows the logic of "screening amplification": first, multiple rounds of cultivation are carried out in a small reactor to screen for effective synthetic products (such as high-quality bacterial strains and high-value metabolites); Gradually scaling up to large-scale reactors to meet industrial production capacity requirements. In addition, the reactors are equipped with interfaces for air supplementation, feeding, acid-base supplementation, and sugar supplementation, which are used for oxygen supply, nutrient supplementation, acid-base adjustment, and carbon source supplementation, respectively, and are coordinated with sensors to form a closed-loop control.
The core value of this tool system lies in reducing experimental costs and improving screening efficiency for small reactors, achieving mass production and implementation for large reactors, ensuring stable cultivation with sensors, and synergistically promoting the industrial application of synthetic biology in the field of high-temperature pharmaceuticals.