Method for testing blood alcohol content

GA/T 842-2019

Release date: April 19, 2019

Implementation date: May 1, 2019

Centralized Unit: National Technical Committee for Standardization of Road Traffic Management

Established headspace gas chromatography analysis method for detecting alcohol (ethanol) content in blood

Qualitative and quantitative analysis of alcohol in personnel's blood during road traffic law enforcement activities

The national standard requires a detection limit of 1mg/100ml for ethanol in blood and a quantification limit of 5mg/100ml

Headspace analysis conditions:

1. Heating box temperature: 65 ℃

2. Quantitative ring temperature: 105 ℃

3. Transmission line temperature: 110 ℃

4. Sample bottle heating equilibrium time: 10.00min

5. Sample bottle pressurization time: 0.1min

6. Quantitative ring filling time: 0.1min

7. Quantitative ring equilibrium time: 0.05min

8. Injection time: 1.00min

Gas chromatograph analysis conditions:

Equipped with a hydrogen flame ionization detector (FID)

Chromatographic column: can refer to standard recommendations

Carrier gas: high-purity nitrogen

Detector temperature: 250 ℃

Injection port temperature: 200 ℃

Column temperature: Different chromatographic column temperatures are set differently

Suggest using a fully automatic headspace sampler in conjunction with it