At the intersection of nanotechnology and biomedicine, a revolution in diagnosis, treatment, and precision drug delivery is accelerating. In recent years, extracellular vesicles - the "messengers" of intercellular communication - have attracted widespread attention from the scientific community. They not only carry rich biological information and participate in intercellular material exchange, but also demonstrate enormous clinical application potential.

However, how to accurately analyze the physical characterization characteristics of extracellular vesicles has always been a challenge faced by researchers. Traditional optical methods are limited by resolution and find it difficult to capture key data at the single particle level. And NanoCoulter based on RPS (Resistance Pulse Induction) principle ™ The nano Coulter particle analyzer, with single particle detection capabilities comparable to electron microscopy, provides a more accurate, sensitive, and convenient characterization method for extracellular vesicle research.

NanoCoulter ™ The technical strength of has received increasing attention in the academic community, and recent research has cited and verified its advantages in extracellular vesicle characterization. This article will list several latest academic papers for everyone to explore how nano Coulter technology can help scientific breakthroughs and open up new paths for nanobiology research!

Application Article 1

This article discussed the potential of Platycodon grandiflorum derived extracellular vesicles (PGEVs) in the treatment of triple negative breast cancer (TNBC). Research has found that PGEVs significantly inhibit the growth of TNBC by inhibiting tumor cell proliferation, inducing cell apoptosis, promoting polarization of tumor associated macrophages (TAMs) towards M1 phenotype, and regulating gut microbiota. PGEVs exhibit good biodistribution and biosafety after oral and intravenous injection, effectively accumulating at the tumor site and significantly improving the therapeutic effect of TNBC by enhancing systemic anti-tumor immune response and regulating gut microbiota. The research results indicate that PGEVs are a natural and biocompatible nanotherapy candidate suitable for the treatment of TNBC.

Using NanoCoulter in the text ™ essenceThe particle size and Zeta potential of PGEVs were accurately characterized, which helped researchers analyze the particle size distribution and surface characteristics of PGEVs, proving that PGEVs have good stability and are suitable as potential drugs for nanotherapy.

Application Article 2

Researchers have delved into the potential of extracellular vesicle like nanoparticles (HELNs) derived from Houttuynia cordata for treating colitis in mice. HELNs significantly alleviated colitis induced by dextran sulfate sodium (DSS) in mice, alleviating colitis symptoms and histopathological damage. In addition, HELNs can specifically target inflamed colon tissue, regulate the immune environment, and alleviate inflammatory responses. Combined with the use of NLRP3 gene knockout mice, RNA sequencing analysis showed that HELNs inhibited the NLRP3/NOD like receptor signaling pathway. Finally, HELNs also balanced the gut microbiota composition of colitis mice, reducing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria in their intestines.

Using transmission electron microscopy (TEM) and NanoCoulter in the article ™ Accurate measurements were made on the particle size and concentration of HELNs, and these characterization results provide fundamental data for subsequent experiments and mechanism studies.

Combining TEM and NanoCoulter ™， Comprehensively characterize HELNs. The particle size distribution in the histogram shows uneven size of extracellular vesicle like nanoparticles, with a wide particle size distribution (ranging from 60-200 nm), which is also reflected in TEM images.

In the control group, the morphology of colon tissue was normal, epithelial cells were arranged in an orderly manner, goblet cells were abundant, mucosal integrity was preserved, and the submucosal layer was discrete; DSS induced colitis in mice presents as severeGoblet cell loss, extensive crypt depletion, significant inflammatory cell infiltration and submucosal thickening in the lamina propria; HELNs significantly reduced DSS induced pathological damage in a dose-dependent manner, with observable crypt glands limited to inflammatory infiltration in the lamina propria without significant mixed infiltration, reduced damaged epithelial cells, and increased goblet cells.

Application Article 3

Application Article 4

Using NanoCoulter in the text ™ The precise characterization of the particle size and Zeta potential of G-Exo helped researchers confirm its particle size distribution and surface properties, demonstrating its good stability and suitability for application in the biomedical field.

Application Article 5

This article introduces a novel chemotherapy drug delivery system, in which researchers use iRGD-TRP-PK1 modified red blood cell membrane vesicles (RBCVs) for targeted treatment of head and neck cancer (HNC). The system achieves targeted drug delivery and release to tumor cells by combining iRGD (a peptide with tumor penetration ability) with TRP-PK1 (a synthetic peptide that can autonomously insert into the cell membrane and form potassium ion channels). The RBCVs modified with iRGD-TRP-PK1 can rupture in the high potassium environment inside tumor cells, releasing the carried chemotherapy drugs, effectively inhibiting tumor growth and reducing toxicity to healthy tissues.

Using NanoCoulter in the text ™ The particle size distribution and Zeta potential of RBCVs were characterized, which are crucial for evaluating the stability of drug delivery systems. With the help of RPS technology, researchers can optimize the preparation process of RBCVs, verify their physicochemical properties, and provide important experimental evidence for their application in treatment.

Through NanoCoulter ™ Characterize the particle size, concentration, and Zeta potential of RBCVs. Significant differences in particle size distribution can be measured in different binding states.

NanoCoulter ™ Not only does it demonstrate accuracy and stability in the application of cell vesicles, but it also plays an important role in the characterization of inorganic nanoparticles in materials science^[1].