Intelligent Artificial Climate BoxIt is a specialized equipment that simulates natural environmental factors such as temperature, humidity, and light, and is widely used in fields such as plant cultivation, seed germination, microbial experiments, and material aging testing. Scientific selection and standardized practical operation can ensure the stability of experimental conditions and the accuracy of data. This article breaks down the specific operation methods from two major modules: the core selection points and the practical operation process.
1、 Key points for selecting intelligent artificial climate chambers
1. Core parameters for matching experimental requirements
Temperature and humidity range and accuracy
Conventional biological experiments: The recommended temperature range is -10 ℃~60 ℃, and the humidity range is 30%~95% RH. The accuracy should reach ± 0.5 ℃ for temperature and ± 5% RH for humidity, meeting the basic requirements for plant growth and bacterial culture.
Special experimental scenario: Low temperature stress experiment can choose the model with the lowest temperature -20 ℃; Equipment with temperature ± 0.1 ℃ and humidity ± 2% RH should be selected for high-precision material testing.
Lighting system configuration
Plant cultivation should pay attention to light intensity (recommended to be adjustable from 0 to 150000 lx) and spectral type: for seedling cultivation, cold white light LED is selected, and for Arabidopsis and other model plant experiments, ultraviolet and far red light modules can be used.
For experiments without light requirements (such as anaerobic cultivation of microorganisms), models with no light function can be selected to reduce procurement costs.
Effective volume and internal layout
Small batch sample experiment: Select a small box of 50~150L, suitable for laboratory countertops; Large scale screening experiment: Select a vertical box with a capacity of 200-1000L, which can be equipped with multiple layers of partitions (≥ 5 layers) to improve space utilization.
Pay attention to the internal dimensions of the box to ensure that it can accommodate experimental containers such as culture dishes, tissue culture bottles, and seedling trays.
2. Pay attention to the intelligent functions and reliability of devices
Intelligent control and data recording
Prioritize models with programmable control systems that support setting multiple temperature, humidity, and lighting programs (such as day night alternation and high/low temperature cycling) to meet the needs of simulating natural environments.
It is necessary to have automatic data recording and export function (USB/Ethernet interface) to facilitate the traceability and analysis of experimental data.
Circulation and ventilation system
Internally, a forced convection fan should be equipped to ensure uniform distribution of temperature, humidity, and light inside the box (uniformity ≤ ± 2 ℃), avoiding local environmental differences that may affect experimental results.
It is recommended to equip the equipment for microbiological experiments with an independent fresh air exchange system to prevent cross contamination of gases inside the box.
Safety protection device
Equipped with over temperature, over humidity, and power-off alarm functions, as well as door safety lock and compressor delayed start protection; Partial experimental scenarios (such as high temperature and high humidity) can be equipped with additional water shortage alarm devices.
3. Adapt to laboratory usage conditions
Installation environment: Confirm that the device size matches the laboratory space and reserve a heat dissipation space of ≥ 30cm; The equipment should be placed on a flat surface, away from heat sources, direct sunlight, and strong electromagnetic fields.
Energy consumption and power supply: high-power equipment (such as those with a capacity of over 500L) needs to confirm the laboratory power supply capacity (recommended 380V three-phase power) to avoid tripping; Long term operation can choose energy-saving compressor models to reduce usage costs.
IIIntelligent Artificial Climate BoxPractical Guide
1. Preparation before the experiment
Equipment inspection and cleaning
Before starting up, check whether the water level in the water tank is sufficient, whether the filter is clean, and whether the door sealing strip is intact; Wipe the partition and inner wall inside the box with 75% alcohol, let it dry before placing the sample to avoid contamination.
Connect the power supply, start the equipment and run it without load for 30 minutes, test whether the temperature, humidity, and lighting system are normal, and confirm that the parameters are stable before proceeding to the next step.
Experimental parameter setting
According to the experimental plan, set the temperature, humidity, light intensity, running time, and program cycle (such as 12h light/12h dark cycle) on the control panel.
Complex experiments can be set up with multiple programs, such as the first stage (0-24h) at 25 ℃, 60% RH, and 50000lx; The second paragraph (24-48 hours) simulates a day night temperature difference environment at 15 ℃, 70% RH, and 0 lx.
Sample placement
Place the experimental samples (culture dishes, tissue culture bottles, etc.) evenly on the partition to avoid blocking the air outlet and ensure smooth airflow circulation inside the box; Sample spacing ≥ 2cm to prevent uneven local environment.
2. Experimental process operation specifications
Real time monitoring and recording
Regularly check the operating status of the equipment during the experiment, record the actual parameters of temperature, humidity, and light; If parameter fluctuations exceed the allowable range, pause the experiment and investigate the cause.
It is strictly prohibited to frequently open and close doors while the equipment is running, and the opening time should be controlled within 1 minute each time to prevent drastic changes in the environment inside the box.
Special case handling
If there is a power outage, the device will automatically save the experimental program and can continue to operate after power is restored; If the power outage time is too long, the sample should be taken out in a timely manner to avoid experimental failure.
If an alarm prompt appears, first check the alarm code (such as over temperature alarm, water shortage alarm), and then take targeted measures (such as adjusting temperature, supplementing water tank water volume).
3. Cleaning and maintenance after the experiment
Sample extraction and cleaning
After the experiment is completed, wait for the equipment temperature to drop to room temperature before taking out the sample; Drain the remaining water from the water tank, clean the water tank and filter with clean water, and store them after drying.
Wipe the remaining stains inside the box with a soft cloth. If there are stubborn stains, neutral cleaning agents can be used. Do not use strong acid or alkali cleaning agents to prevent corrosion of the box.
Key points of daily maintenance
Regularly replace the filter: Clean or replace the air filter every 1-3 months to prevent dust from clogging and affecting ventilation.
Compressor maintenance: When not in use for a long time, disconnect the power supply and keep the box dry; Start up and run once a month (30 minutes) to avoid compressor aging.
Sealing strip inspection: Check the elasticity of the door sealing strip every six months. If aging or cracking occurs, replace it in a timely manner to ensure the sealing of the box.