Serum-free culture mediumReagents are widely used to cultivate mammalian and invertebrate cells for the preparation of monoclonal antibodies, viral antigens, and recombinant proteins. Most serum-free culture media contain transferrin, which transports ions into cells, insulin, which regulates glucose uptake, as well as proteins such as albumin, fibrin, and fetoprotein. These proteins play various roles in cell culture, such as providing the matrix required for cell adhesion, resisting bioreactor shear forces, and serving as carriers for lipids and other growth and differentiation factors.
Stem cell culture medium is a type of mesenchymal stem cell (hMSC) culture medium that has a clear chemical composition, is serum-free, and does not require a substrate. This culture medium can be used for primary cell isolation and cell passage of human mesenchymal stem cells from various sources, while maintaining their potential for multi-directional differentiation, such as bone marrow (BM hMSC), adipose tissue (AT hMSC), and umbilical cord (UCM hMSC). There is no need to add serum or serum substitutes when using this product, and the batch differences are small, resulting in a fast cell doubling rate. Its core components include:
Basic culture medium: containing essential amino acids, vitamins, and energy substances such as glucose and glutamine.
Supplementary factors: such as recombinant proteins (transferrin, insulin), growth factors (FGF, EGF), and trace elements (selenium).
No animal derived additives: Replace traditional serum with human or synthetic substitutes (such as recombinant laminin).
Safety: Serum free culture medium - avoids the risk of pathogen contamination and reduces immune rejection reactions; Serum culture medium - containing heterologous animal components, potential allergen risk.
Batch consistency: Serum free culture medium - clear chemical composition, high experimental reproducibility; Serum culture medium - serum composition is complex and varies greatly between batches.
Cell performance: Serum free culture medium - maintains stem cell stemness and low differentiation rate; Serum culture medium - easy to induce differentiation, with flattened cell morphology.