The ex vivo tissue tension perfusion system is a widely used device in biomedical experiments, used to simulate the in vivo environment and study the physiological functions and pharmacological properties of ex vivo tissues (such as blood vessels, smooth muscles, myocardium, etc.). The system maintains the biological activity of ex vivo tissues by providing a constant temperature environment, nutrient solution circulation, and oxygen supply, and measures the tension changes of tissues in real time under the action of drugs or electrical stimulation.
1、 Technical core functions
Constant temperature environment control
The system adopts electric heating PID temperature control technology, with a temperature control range of room temperature to 45 ℃ and an accuracy of ± 0.1 ℃. It does not require an external water bath to avoid scale problems.
Compact design saves laboratory space, such as the KW-400D equipment which can save 60% of space.
Tension measurement and data acquisition
High precision tension sensor (0.01g level) measures tissue tension in real time, dynamically draws curves, and provides accurate and reliable data.
Support multi-channel independent measurement (such as 4-channel), and the temperature of each channel can be independently adjusted to meet different experimental needs.
Fluid circulation and gas supply
Intelligent peristaltic pump automatically replenishes/discharges fluids (flow rate 70ml/min) to ensure the circulation of nutrient solution.
Independent air inlet and micro needle valve, adjustable oxygen or experimental gas flow rate to maintain tissue activity.
Experimental flexibility
Built in electrical stimulation electrodes, supporting bidirectional specimen adjustment (± 80mm), suitable for various muscle tissues ranging from 1-35mm.
Detachable bath design, more intuitive top-down operation, convenient for operation under a microscope.
2、 Technical advantages
Efficient automation
Intelligent peristaltic pump automatically replenishes/discharges fluids, reduces manual operation, and improves experimental efficiency.
Automated design throughout the entire process to reduce human error.
High precision and stability
0.01g level tension sensor and 0.1 ℃ temperature control accuracy ensure the reliability of experimental data.
Four channel independent temperature control to avoid interference between channels.
Flexibility and Scalability
Suitable for various tissue samples (such as vascular rings, muscle strips, trachea, intestinal tract, etc.).
The bath capacity, number of specimen hooks, and tension transducer configuration can be customized according to experimental needs.
Maintain convenience
Detachable bath design for easy cleaning and disinfection.
Made of metal and stainless steel pillars, durable and easy to maintain.
3、 Application scenarios
Physiological research
Study the contraction and relaxation mechanisms of smooth muscles such as uterus, blood vessels, trachea, and intestinal tract.
Explore the effects of neurotransmitters (such as NO, H2S) and ion channels on tissue tension.
pharmacological research
Evaluate the mechanism of action of drugs on ex vivo tissues and draw dose-response curves.
Screening vascular active drugs to support new drug development.
Pathophysiological research
Simulate the pathological process of cardiovascular diseases such as hypertension and atherosclerosis.
Study the effects of aging, metabolic disorders, and other factors on tissue function.
Electrophysiological stimulation experiment
Study the effect of electric field on tissue tension through built-in electrical stimulation electrodes.
Explore the relationship between neural signal transmission and muscle contraction.
4、 Typical equipment parameters (taking KW-400D as an example)
Number of channels: 4 independent systems
Bath capacity: 12ml
Tension range: 100g
Temperature control range: Room temperature -45 ℃ (± 0.1 ℃)
Supply voltage: 100-240V wide voltage
Size: Compact body, saving 60% laboratory space
5、 Technological development trends
Intelligence and integration
Combining IoT technology to achieve remote monitoring and automatic data analysis.
Integrate more physiological parameter measurement functions (such as pH value, conductivity, etc.).
Miniaturization and High Throughput
Develop miniaturized bath tanks to support simultaneous measurement of more samples.
Improve experimental throughput and accelerate drug screening process.
multimodal fusion
Combining optical imaging technology to observe real-time changes in tissue morphology.
Integrate electrophysiological signal recording and conduct in-depth research on tissue functional mechanisms.
VI. Summary
The ex vivo tissue tension perfusion system provides powerful technical support for physiological, pharmacological, and pathophysiological research through precise temperature control, high-sensitivity tension measurement, and flexible experimental design. With the continuous advancement of technology, this system will play a more important role in biomedical research, promoting scientific discoveries and clinical applications in related fields.