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Portable fluorescence analyzer can detect very trace amounts of sample components
Date: 2025-11-16Read: 0
Fluorescence analysis is an analytical method based on the fluorescence characteristics of substances. When a substance absorbs light of a certain wavelength, it emits light longer than the excitation light, which is called fluorescence. A fluorescence analyzer determines the properties or concentration of a substance by detecting and analyzing the fluorescence signal emitted by the substance. In portable fluorescence analyzers, lasers or other light sources are used to excite molecules or atoms in the sample into excited states. Excited substances emit light at different fluorescence wavelengths, and the instrument collects and analyzes these emitted fluorescence signals through an optical system to obtain information on the composition or concentration of the sample.
There are usually two basic methods for fluorescence analysis: fluorescence intensity method and fluorescence attenuation method. The fluorescence intensity method quantitatively analyzes substances by measuring the fluorescence intensity emitted by the sample, while the fluorescence decay method studies the molecular dynamic properties of substances by measuring the decay process of the fluorescence emission intensity of the sample over time.
Portable fluorescence analyzerStructure and working principle:
1. Excitation source: The laser or LED light source built into the instrument emits light of a specific wavelength onto the sample. The selection of light source is based on the excitation wavelength of the analyzed substance.
2. Optical system: The excitation light is guided to the sample through optical fibers or lenses, and the fluorescence light signal emitted by the sample is collected. Typically, optical systems include filters to select the appropriate wavelength range, transmitting only fluorescent signals while filtering out stray light.
3. Detector: After passing through the optical system, the fluorescent signal is received by a photodetector (such as a photodiode, photomultiplier tube, etc.) and converted into an electrical signal. The intensity of these electrical signals is directly proportional to the fluorescence intensity.
4. Signal processing and display: The processed signal will be displayed on the screen, and the analysis results can be directly displayed as fluorescence intensity, concentration, or other related data.
Features:
1. High sensitivity: able to detect very small amounts of sample components. Fluorescence signals are extremely sensitive to low concentrations of substances, so even trace amounts of substances can be accurately detected.
2. Quick analysis: can provide test results within seconds or minutes, especially suitable for situations that require immediate feedback.
3. Easy to operate on site: Due to its compact size, it can be easily carried to the site for testing and is widely used in fields such as field environmental monitoring, food testing, medical diagnosis, etc.
4. No need for complex sample pretreatment: Compared with other analytical methods, fluorescence analysis usually does not require complex sample pretreatment steps, which greatly improves detection efficiency and accuracy.
5. High selectivity: Fluorescence analysis can selectively analyze target substances in complex samples, avoiding interference and errors.
6. Multifunctionality: Often equipped with multiple functions, it can analyze multiple substances and perform multi parameter detection by replacing different excitation light sources and filters.
Application areas of portable fluorescence analyzer:
1. Environmental monitoring: Its application in environmental monitoring is particularly important. It can be used for water quality monitoring, air quality testing, soil pollution assessment, etc. For example, by measuring the concentration of organic pollutants (such as polycyclic aromatic hydrocarbons, heavy metal ions, etc.) in water, the water quality condition can be evaluated.
2. Food safety testing: In the food industry, it is used to detect harmful substances in food, such as pesticide residues, additives, microbial contamination, etc. It can quickly detect toxic substances in food, ensuring food safety.
3. Medical diagnosis: In the field of medicine, it can be used for the detection of biomarkers in body fluids. By detecting specific molecules (such as viruses, bacteria, hormones, etc.) in blood, urine, and other samples, clinical diagnosis and treatment decisions can be assisted.
4. Chemical analysis and research: used in chemical laboratories for qualitative and quantitative analysis of substances. Its high sensitivity and selectivity make it an ideal tool in chemical experiments.
5. Military and security testing: It can also be applied in the military and security fields to detect hazardous substances, explosives, etc. It plays an irreplaceable role in the rapid detection and prevention of dangerous goods.