The HILICpak VG-50 4E polymer amino column can meet the requirements of this national standard for chromatographic columns (amino chromatographic column, column length 250 mm, inner diameter 4.6 mm, particle size 5 μ m, or chromatographic column with equivalent performance). Using VG-504E in mobile phase CH₃CN/H₂O=88/12， Temperature 60˚ Under the condition of C, all sugars can be eluted, and the separation degree between each sugar is greater than 2.5, far higher than the requirement of separation degree greater than 1.5 in the national standard. The detection limit of each sugar is less than 0.002 g/100 g, far below the requirement of 0.2 g/100 g in the national standard. The RSD of 6 repeated experiments is less than 3%, far below the requirement of 10% in the national standard. There may be differences in separation degree, detection limit, and precision among different high-performance liquid phase systems.


Sample : 6 mg/mL each, 5 µL
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose

Column       : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)Eluent       : CH3CN/H2O=88/12Flow rate    : 1.0 mL/minDetector     : (A);  RI (40 ℃), (B);  ELSD (80 ℃)Column temp. : 60 ℃