Firstly, according to relevant standards such as YY/T1503, ASTMF382, etc., prepare test samples, usually representative finished titanium alloy bone plates. Install the bone plate on a bending fatigue testing machine equipped with a four point bending fixture, ensuring that the fixture is correctly calibrated and that the portion of the bone plate typically connected to the fracture site is subjected to uniform bending moments.

Next, set the experimental parameters, including frequency, load ratio, and testing period, to simulate common biomechanical environments and ensure that the specimen is subjected to moderate stress. Generally, a fixed frequency sinusoidal cyclic four point bending load is applied to the bone plate to initiate fatigue testing. During the testing process, start the testing machine to load the bone plate and continuously monitor the changes in load and displacement, while recording the test data and specimen status.

Regularly inspect the surface and structure of the bone plate to observe for cracks or other damages. When the sample fractures or the number of cycles reaches the set termination number, the test stops. The termination frequency is pre-set for specific samples. If the purpose of the experiment is to determine the fatigue strength at a specific number of cycles, the termination frequency is usually specified as that number of cycles.

Finally, analyze the data collected during the testing period, including the number of experiments, load displacement curves, etc. Draw a stress life curve (S-N curve) based on the data, determine the fatigue limit, and evaluate the fatigue life of the bone plate. By analyzing the causes and methods of sample failure, such as observing fracture morphology and analyzing residual stress, we can further understand the fatigue performance of titanium alloy plates, provide important references for the design and improvement of medical devices, and ensure that titanium alloy plates have sufficient fatigue life and reliability in practical applications.