Pharmaceutical analysis and detection instruments require only the sample to be able to form a solution through liquid chromatography, without being limited by the volatility of the sample. The range of mobile phases available is wide, and there are many types of stationary phases, which can separate thermally unstable and non-volatile, dissociated and non dissociated, and substances with various molecular weight ranges. Combined with sample pretreatment techniques, the high resolution and sensitivity achieved by HPLC make it possible to separate and simultaneously determine substances with similar properties, enabling the separation of trace components in complex phases. With the development of stationary phases, HPLC has become the most promising method for solving biochemical analysis problems while maintaining the activity of biochemical substances. Due to its high resolution, sensitivity, fast speed, reusable chromatographic columns, and easy collection of effluent components, HPLC is widely used in various fields such as biochemistry, food analysis, pharmaceutical research, environmental analysis, and inorganic analysis.

The combination of pharmaceutical analysis and testing instruments with structural instruments is an important development direction. The liquid chromatography-mass spectrometry technology has received widespread attention, such as the analysis of carbamate pesticides and polycyclic aromatic hydrocarbons; The combination of liquid chromatography and infrared spectroscopy has also developed rapidly, such as the determination of hydrocarbons in water and non-volatile hydrocarbons in seawater for environmental pollution analysis, which has brought new developments to environmental pollution analysis.