Digital portable automatic colorimeter is an instrument used to measure the color change and concentration of solutions, widely used in environmental monitoring, water quality testing, food industry, chemical analysis and other fields. Its working principle is usually calculated by colorimetric method based on the relationship between the absorbance and concentration of the target substance in the solution. The following is the general usage process of a digital portable automatic colorimeter:
Usage process of digital portable automatic colorimeter
preparation:
Check the status of the instrument: Ensure that the colorimeter battery is sufficient, the power switch is normal, and the instrument is not damaged.
Choose a suitable colorimetric tube/cuvette: Use a clean colorimetric tube or cuvette to avoid contamination of the light path. Most colorimeters are equipped with standard colorimetric tubes.
Choose colorimetric reagents: Prepare appropriate chemical reagents according to the substance to be measured. Colorimetric reagents are selected based on the reaction characteristics (such as color changes) of the solution being tested.
Instrument calibration:
Selecting standard samples: Typically, instruments are calibrated using standard solutions of known concentration or color.
Perform zero point calibration: Fill the colorimetric tube with pure solvent (such as deionized water or standard solution) and place it in the colorimeter for zero point calibration. The instrument will automatically adjust to ensure accurate measurements in the future.
Select wavelength: Some instruments support manual wavelength selection or automatically detect the optimal wavelength. The common wavelengths range from 400nm to 800nm, depending on the substance being measured and its absorption characteristics.
Sample preparation:
Sampling: Take the sample solution to be tested into a colorimetric tube, ensuring that the sample volume is appropriate and evenly mixed.
Add reagents: According to the analysis requirements, add appropriate colorimetric reagents to ensure thorough mixing. If the sample needs to react for a certain period of time, wait as required.
Measurement sample:
Put the prepared sample solution into the colorimetric tube, ensuring that there are no bubbles.
Measurement process: Place the colorimetric tube into the optical path of the instrument, ensuring alignment with the optical path and photosensitive components. The instrument will illuminate the sample with a light source, measure the absorption of specific wavelengths of light by the sample, and then calculate the concentration or color value of the sample.
Reading results: The instrument will automatically display the measurement results, usually digital concentration values, absorbance, or other related parameters. Some instruments may provide graphical output or store measurement history data.
Recording and analysis:
Save data: Many portable colorimeters can store measurement results inside the instrument or export data through USB interfaces, Bluetooth, and other methods.
Analysis results: Based on the measurement results, combined with the preset standard curve or concentration absorbance relationship, analyze the concentration or color change of the sample.
Cleaning and maintenance:
Clean the colorimetric tube: After the measurement is completed, immediately clean the colorimetric tube to prevent residue from affecting the next measurement.
Check the instrument: Regularly inspect the light source, optical components, display screen, etc. of the instrument to keep it in good condition.
Notes:
During the measurement process, it is important to ensure that there are no bubbles in the sample, as bubbles can affect the propagation of light and result in inaccurate measurement results.
When using reagents, pay attention to their storage and expiration dates, and avoid using expired reagents.
Ensure that the same type of colorimetric tube and solvent are used for each measurement to reduce the influence of external variables.
In strong lighting environments, it may be necessary to use a light shield or operate the instrument in a dark place to improve measurement accuracy.
Through the above steps, the digital portable automatic colorimeter can quickly and accurately measure the color of samples, and is widely used in laboratory analysis, environmental monitoring, and other fields.