The vibrating circular dichroism spectrometer can accurately analyze samples at low concentrations and even detect samples with micromolar concentrations. This is particularly important for trace analysis and research on biomolecules, as it can obtain reliable data without damaging the sample. Unlike some traditional techniques that require staining or fluorescent labeling, VCD technology directly utilizes the optical activity of the molecule itself for measurement, without the need for any external labeling or chemical modification of the sample. This not only simplifies the experimental steps, but also reduces potential interference factors and improves the authenticity of the results.

This instrument can not only analyze samples in solution, but also analyze thin films, fibers, or other solid samples. This wide applicability makes VCD technology have broad application prospects in multiple fields such as materials science and biology. In addition to providing static structural information, it can also monitor the dynamic changes of molecular structure in real time. The use of polarization modulation system and algorithm optimization technology effectively eliminates false signal interference and improves the reliability of detection results. For example, using ZnSe material modulator and high-frequency modulation of polarized light reduces the impact of thermal effects on measurement; Meanwhile, by combining hardware improvements with software algorithms, the signal-to-noise ratio and stability have been further improved.

Precautions for using a vibrating circular dichroism spectrometer:

1. In terms of samples

-Absorption range control: The infrared absorption peak absorbance value of the sample should be between 0.2-0.8 and not exceed 1.0. Too low can cause weak VCD signals, while too high can result in false noise signals in the VCD signal. If there is a significant difference in the absorption intensity of the infrared absorption peak of a compound, it can be measured twice with high and low concentrations to ensure that the absorbance falls within the appropriate range.

-Purity requirement: Ensure that the sample purity is high enough, without impurities or other contaminants, to avoid affecting the quality of the test results.

2. Operating standards

-Follow the operating procedures: Operators are not allowed to enter programs outside the operating procedures at will, and are not allowed to press function keys outside the rules to prevent equipment failure caused by misoperation.

-Proper placement of sample holder: The sample holder should be pressed vertically, and the excitation button should not be pressed when the sample is not properly clamped. The sample holder should not be touched or lifted during excitation; If the sound is abnormally loud during excitation, press the F3 key to stop. Do not lift the sample holder directly, otherwise it may burn the circuit board.

-Be careful when disassembling components: When removing the spark table, handle it gently to prevent scratches on the inner surface; Be careful when removing the circular quartz gasket and glass sleeve from the spark chamber to avoid breakage.

3. Daily maintenance and safety

-Regularly clean the spark table: The black deposits generated after exciting the sample may cause a short circuit between the electrode and the spark table, so it is necessary to clean the spark table regularly.

-Timely reporting of fault handling: Once the instrument malfunctions or exhibits abnormal phenomena, the power should be immediately turned off to protect the site and promptly report to technical personnel for handling.

-Power outage response measures: In the event of a sudden power outage, turn off the main power first, and then turn off other power sources; After receiving the call, follow the procedure to turn on the device.