The electrophysiological perfusion instrument can accurately control various parameters of perfusion, including minimal flow fluctuations, which is crucial for studying bioelectrical activity at the microscale. It can be used in conjunction with microscopes, electrodes, and other measuring devices to achieve multidimensional observation and detection. This integration capability enables researchers to record the electrophysiological signals of cells while observing their morphology, providing strong support for further research on cell function.

Some have fast solvent switching function, no dead volume, and no cross contamination between solvents, which can quickly allow cells exposed to solvent flow to reach equilibrium in the perfused solvent, greatly improving experimental efficiency and shortening the experimental period. Whether it is single-cell recording, tissue level research, or even organ perfusion experiments, they can all play an important role. The dual channel system can be easily expanded to multiple channels to meet experimental design requirements of different levels of complexity; The sturdy horizontal tissue bath is suitable for combining with electrophysiological research on the same platform for intracellular electrical signal recording.

Platinum black electrodes allow cells to be directly cultured on the electrode surface, combined with a constant temperature perfusion system, which can meet the needs of long-term continuous observation and recording, suitable for studying slowly changing physiological processes or long-term drug effects. By regularly calibrating equipment and maintaining it, the accuracy and stability of measurement results are ensured, providing a reliable data foundation for researchers and facilitating the drawing of scientifically reasonable conclusions.

Measurement steps of electrophysiological perfusion instrument:

1. Patient/sample preparation

-Evaluation and communication: If human experiments are involved, the patient's clinical history needs to be evaluated first to ensure safety; Explain the operation process and possible feelings to the patient, and answer any questions. For animal experiments, appropriate anesthesia methods and fixation devices (such as scaffolds) need to be selected based on the species.

-Cleaning treatment: Clean the skin or sample surface in the measurement area to ensure good electrode contact. For example, in ex vivo brain slice experiments, excess tissue fluid needs to be removed to enhance signal stability.

2. Electrode placement and connection

-Attach electrodes: Place the electrodes accurately on the target area (such as the chest, limbs, etc.), depending on the type of experiment. In vitro organ experiments often use microelectrodes inserted into specific areas.

-Secure connection: Ensure stable connection between all electrodes and instruments to avoid data interruption or noise interference caused by looseness.

3. Parameter setting and system debugging

-Basic configuration: Set parameters such as perfusion speed, temperature, and gas concentration (such as oxygen/carbon dioxide ratio) to simulate physiological environments. For example, cardiac perfusion can use constant pressure mode to maintain a constant pressure.

-Calibration verification: Check whether the actual value matches the preset range through flow meters, pressure sensors, and temperature probes, and control the error within the allowable range. Simultaneously check the working status of the pump and the sealing of the system to prevent leakage from affecting the results.

4. Real time monitoring and recording

-Dynamic observation: Continuously monitor changes in electrophysiological signals after activation, including electrocardiogram, action potential duration, conduction velocity, and other indicators. Combining microscopy or other imaging techniques to assist in locating areas of abnormal activity.

-Data collection: Utilizing integrated amplifiers and software tools to synchronously record multi-channel data for subsequent analysis and comparison of differences under different conditions.

5. Experimental termination and subsequent processing

-Orderly shutdown: After completing the measurement, gradually stop the equipment operation and gently remove the electrodes to avoid mechanical damage. Fixed storage or further processing of ex vivo samples.

-Data analysis: Using professional software to analyze signal characteristics, identify abnormal patterns, and associate them with pathological mechanisms to provide a basis for research conclusions.