18 angle laser light scattering instrumentMALLS is an advanced analytical instrument based on the principle of laser scattering, which can directly and accurately measure the absolute molecular weight and molecular size of compounds in solution. The operation process includes standardized steps such as calibration, sample preparation, instrument setup, data acquisition and processing.

1、 Calibration process

Select calibration samples: Select samples with known molecular weight and weight average molecular weight as calibration samples to ensure the accuracy of instrument measurements.

Prepare calibration solution: Dissolve a sample with a known molecular weight in a suitable solvent and prepare a solution of a certain concentration.

Start calibration: Place the calibration solution into the instrument and calibrate according to the instrument's operating instructions. Usually multiple scans are required to record the scattered light intensity and angle distribution.

Data processing and analysis: Based on the calibration results, analyze the measurement errors and uncertainties of the instrument to evaluate its accuracy.

2、 Sample preparation

Select appropriate samples: Ensure that the samples have high scattered light intensity in order to obtain clear scattered signals.

Clean the surface of the sample: to prevent impurities from interfering with the measurement results.

Sample processing: The sample and solvent need to be strictly filtered to remove particle impurities.

3、 Instrument settings

Preheating instrument: Turn on the power switch of the multi angle laser light scattering instrument and wait for the instrument to preheat for about 15 minutes to ensure that the instrument reaches a stable working state.

Adjust gain and sensitivity: Ensure good signal capture performance.

Set measurement parameters:

Measurement wavelength and angle range: Select the appropriate measurement wavelength and angle range to obtain the desired scattered light data.

Grating angle and spectral bandwidth: Adjust the parameters of the light scattering instrument, such as grating angle, spectral bandwidth, etc., to optimize the measurement effect.

4、 Data collection

Place the sample: Place the sample in the appropriate position to ensure that the distance between the sample and the light source is appropriate. Adjust the angle and position of the sample to be perpendicular to the light source and maximize the reception of scattered light.

Start measurement: Click the "Start Measurement" button, and the light scattering instrument will start recording scattered light data. During the measurement process, avoid moving or touching the instrument and sample.

Waiting for measurement completion: Save the measurement results and export the scattered light data to the data processing software in the computer.

5、 Data Processing and Analysis

Data fitting and analysis: Based on the experimental purpose, select appropriate data processing methods to fit, analyze, or compare scattered light data to obtain the required relevant parameters.

Create charts or images: Display experimental and analytical results, and use appropriate legends and labels to ensure that the images are clear and easy to understand.

Summary of experimental results: Analyze the consistency or differences between experimental results and expected results, and explore possible sources of error in the experiment. Propose improvement measures or suggestions for further research, and briefly review the significance and importance of the experiment.

VI18 angle laser light scattering instrumentInstrument maintenance and upkeep

Regularly clean the instrument: Clean the inside and outside of the multi angle laser light scattering instrument to remove dust and dirt. Be careful not to damage the optical components inside the instrument during the cleaning process.

Check optical components: Regularly inspect optical components for scratches, fingerprints, or other defects. If necessary, damaged optical components can be replaced.

Keep the interior of the instrument clean: Use dry nitrogen or inert gas to clean the interior of the instrument to remove moisture and oxygen, and keep the interior of the instrument dry.

Check the laser source: Regularly check whether the power and wavelength of the laser source are stable, and replace the laser source if necessary.

Regular maintenance: According to the recommendations of the instrument manufacturer, regularly maintain and upkeep the instrument to ensure its long-term stability and accuracy.