Since the discovery of novel coronavirus, especially the nucleic acid detection of novel coronavirus in PCR laboratory, the task is arduous. First, let me introduce the process of PCR detection:

1Reagent preparation area

Function: It is mainly used for the preparation of storage reagents, the packaging of reagents, and the preparation of main reaction mixtures. Reagents and materials used for specimen preparation should be transported directly to this area and should not pass through other areas. Reagent raw materials need to be stored in this area and prepared into the required storage reagents within this area. Required instruments and equipment:

balance(Used for precise weighing of various reagents)clean bench(All operations involving bacteria must be completed under an ultra clean workbench)pipette(Accurately measure 20-100ul of a specific volume of solution)pure water(Ultra pure water 18.2 Ω M.CM)Medicine Refrigerator（短期保存）Reagent low-temperature refrigerator（Optional 2-8 ° C, -20 ° C）Mixing device(Mixing is required before transferring the sample) (Recommended brand: MixMax vortex mixer)Disinfection vehicle(Space environment disinfection)sterilization pot(Disinfection and sterilization of laboratory items, reagents, and culture media)PHplan(The configuration of reagents is for precise measurement of pH value)

2 Sample preparation area

Function: Preservation of clinical specimens, extraction and storage of nucleic acids (RNA, DNA), addition to amplification reaction tubes, and synthesis of DNA during RNA measurement. Required instruments and equipment

biosafety cabinet(To prevent the spread of harmful suspended particles and aerosols)Sample low-temperature refrigerator(Store samples and DNA at -20 ℃, -80 ℃)High speed desktop freeze centrifuge(Used for collecting microbial cells, cell fragments, and other sediment. Some samples require a low-temperature environment due to their instability at room temperature.)Constant temperatureMetal bath(Used for water bath temperature control during DNA hybridization process) (HtPot40 constant temperature metal bath)pipette(Accurately measure a specific volume of solution)UV lamp or disinfection vehicle(Space environment disinfection)mixHomogenizer(Mixing is required before transferring the sample) (Recommended brand: MixMax vortex mixer)clean bench(All operations involving bacteria must be completed under an ultra clean workbench)Low temperature shaker(Used for shaking culture of Escherichia coli and yeast)Magnetic Stirrer(Accelerated dissolution of solid contents

3Nucleic acid amplification zone

Function: It mainly involves DNA amplification, the preparation of prepared DNA templates and synthesized DNA, and the preparation of reaction mixtures. It can also be carried out in this area. In PCR assays, it is usually necessary to open the reaction tube after amplification.

Gene amplifier(Gene testing DNA/RNA amplification)Real time PCR(Nucleic acid quantitative analysis)Nucleic acid extraction instrument(Sample DNA/RNA extraction)pipette(Accurately measure a specific volume of solution)UV lamp or disinfection vehicle (Space environment disinfection)clean bench(All operations involving bacteria must be completed under an ultra clean workbench)Water purifier(Used for PCR, DNA sequencing requires ultrapure water)

4Product analysis area

Function: The main operation is the determination of amplified fragments. If using a fully automatic closed analysis instrument for detection, the following equipment is required:

Gene amplifier (Gene testing DNA/RNA amplification)Real time PCR(Nucleic acid quantitative analysis)Nucleic acid extraction instrument(Sample DNA/RNA extraction)pipette(Accurately measure a specific volume of solution)UV lamp or disinfection vehicle (space environment disinfection)Ultra clean workbench (all operations involving bacteria must be completed under the ultra clean workbench)Water purifierUltra pure water is required for PCR and DNA sequencing

electrophoresis apparatusHorizontal electrophoresis is used for nucleic acid DNA/RNA detection, vertical electrophoresis is used for protein detection, and transfer electrophoresis is used to transfer proteins onto membranes for western detectionGel imaging system/UV detector(Used for observation and photography of nucleic acid agarose electrophoresis and protein polyacrylamide, UV analyzer is used for observation of stained nucleic acid agarose gel, cannot be connected to a computer)electric furnace(Electrophoresis agar gel heated and melted)