The gel purification system is an important tool for separating and purifying biological macromolecules and other substances. It is mainly based on the principle of gel permeation chromatography to separate and collect complex samples according to the size of molecular volume.
When the mixture passes through a column filled with a specific gel, the speed of molecules moving in the gel will be different according to their size and shape. Smaller molecules can enter the pores of the gel and remain in the column for a long time, while larger molecules are excluded and quickly pass through the column with the mobile phase, thus realizing the separation of different molecules.
Main components
Chromatographic column: Filled with gel material, it is a key component for separating samples. Different gel materials are suitable for sample separation in different molecular weight ranges.
Sample injection device: Ensure that the sample enters the chromatographic column uniformly, usually including a syringe, injection valve, etc.
Mobile phase system: responsible for transferring samples and buffer solutions to the chromatographic column, with some systems having an automatically adjustable mobile phase.
Detector: Used to monitor various components in the effluent, commonly used detectors include ultraviolet detectors and fluorescence detectors, which can detect substances with ultraviolet absorption or fluorescence characteristics in the sample.
Data processing system: used for analyzing and displaying detection data, such as computer analysis software used in high-performance liquid chromatography systems, which can record, analyze, and process detection results.
Technical Features
Effective purification: Separation of target components from matrix is achieved through molecular volume differences, effectively removing large molecule matrix and small molecule interfering substances.
Improving analysis accuracy: The purified sample enhances the sensitivity and accuracy of subsequent analysis, extending the lifespan of the analytical instrument.
High degree of automation: The fully automatic 3D liquid processing function supports immersion cleaning and spacer puncture technology, reducing cross contamination and solvent evaporation.
Flexible purification program: supports six types of fraction collection modes (such as collecting by time, peak, voltage, and their pairwise combinations) to meet different experimental needs.
Strong compatibility: Can be connected online with fully automatic quantitative concentration systems and solid-phase extraction systems, with strong scalability.
Easy to operate: The software versions can be freely switched between Chinese and English, and the graphical interface makes the operation more intuitive and convenient.