1、 Overview of Vitamin A, D, and E and Reasons for Deficiency Status

Vitamin A (retinol): maintains visual function, skin and mucosal integrity, and promotes immune function.

Vitamin E (tocopherol): maintains fertility function, has antioxidant effects,Alpha tocopherol is the main active form.

Vitamin D (sterol derivatives): maintain calcium and phosphorus stability, affect immunity, tumor, diabetes, etc,25 hydroxyvitamin D (250HD) is the optimal indicator of nutritional status.

Lack of current situation: 106 common diseases worldwide are related to insufficient vitamin intake;The vitamin D deficiency rate among children aged 3-5 in China is about 51.9%, the vitamin A deficiency or insufficiency rate exceeds 50%, and the vitamin E deficiency rate is 0.6%.

Lack of reasons: insufficient dietary intake; Fat absorption disorder; Increased demand for the body; Drug interference with metabolism.

2、 Clinical significance and target population of testing

Clinical significance: Nutritional evaluation and dietary guidance before and during pregnancy; Malnutrition in children is often accompanied by deficiencies in multiple vitamins; The prevention and treatment of osteoporosis should pay attention to vitamin D; scientific supplementation should be taken to avoid poisoning (fat soluble vitamins are prone to accumulation).

Target audience:Obstetrics and Gynecology: Pregnancy preparation, lactation, menopause, polycystic ovary, anemia, pregnancy induced hypertension/diabetes, etc.pediatricsPreschool/school aged children, recurrent respiratory infections, weakened immunity, developmental disorders, etc.Other departmentsOsteoporosis, malabsorption, taking lipid-lowering drugs, radiotherapy and chemotherapy, obesity, sub-health, etc.

3、 One stop detection solution

• Testing reagents (NMPA certified):

• Vitamin A/E Determination Kit (Liquid Chromatography Method)

• 25 hydroxyvitamin D determination kit (liquid chromatography method)

• Adaptation Platform: Liquid Chromatography Detection System

• Service support: 24-hour technical support

4、 Advantages of Liquid Chromatography (HPLC)

Liquid chromatography vs. chemiluminescence analysis (CLIA)

1. High sensitivity of HPLC: The sensitivity of HPLC is at the ng level, which can basically meet the determination needs of most biomarkers. CLIA has lower sensitivity for compounds with low detection limits.

2. High specificity of HPLC: HPLC uses physical methods to separate biomarkers, avoiding cross reactions caused by non-specific binding of CLIA antigens and antibodies.

3. High flexibility of HPLC: Experimenters can build their own methodological determination of biomarkers for scientific research on the HPLC platform, which is efficient and convenient. However, developing new biomarker determination kits on the CLIA platform is difficult, time-consuming, and costly.