chromatographic peak

Retention time of separated compounds

1. Habaside 13.661

2. Habaroside 27.702

Chromatographic conditions

Mobile phase:

A: Acetonitrile

B: 0.03% phosphoric acid solution

Gradient program

Time (min) Mobile phase A (%) Mobile phase B (%)

0-10 3-10 97-90

10-20 10-33 90-67

20-25 33-50 67-50

25-30 50-80 50-20

30-35 80 20

35-37 80-3 20-97

Column temperature: 35 ° C

Detector: UV detector

Wavelength: 210nm