chromatographic peak

Retention time of separated compounds

1. Tianma extract 12.106

2. Hydroxybenzyl alcohol 21.501

Chromatographic conditions

Mobile phase:

A: Acetonitrile

B: 0.1% phosphoric acid solution

Gradient program

Time (min) Mobile phase A (%) Mobile phase B (%)

0-10 3-10 97-90

10-15 10-12 90-88

15-25 12-18 88-82

25-40 18 82

40-42 18-95 82-5

Column temperature: 30 ° C

Detector: UV detector

Wavelength: 220nm