chromatographic peak

Retention time of separated compounds

1. Qianghuol 32.007

2. Isoquercetin 48.204

Chromatographic conditions

Mobile phase:

A: Acetonitrile

B: 0.1% phosphoric acid solution

Gradient program

Time (min) Mobile phase A (%) Mobile phase B (%)

0-6 48-53 52-47

6-12 53 47

12-20 53-80 47-20

20-30 80 20

Column temperature: 25 ° C

Detector: UV detector

Wavelength: 246nm