What are the applicable ranges of carbon dioxide constant temperature oscillation shaker?
Date: 2025-11-10Read: 2
The applicable range of carbon dioxide constant temperature oscillation shaker is focused on the needsConstant temperature, constant CO ₂ concentration+dynamic oscillationThe live culture scenario covers multiple research fields such as cell biology, microbiology, and biopharmaceuticals, as follows:
- Suspension cell culture: For the proliferation culture of mammalian suspension cells such as HEK293, CHO, Jurkat, etc., oscillation can ensure sufficient oxygen, uniform nutrition, and support high-density cell growth.
- Hybridoma cell culture: used for monoclonal antibody preparation, oscillating environment can enhance hybridoma cell activity, promote antibody secretion and accumulation.
- Dynamic cultivation of stem cells: such as the expansion of mesenchymal stem cells and embryonic stem cells, gentle oscillation can simulate the mechanical microenvironment in vivo, maintain stem cell stemness or induce differentiation.
- Mild oscillation culture of adherent cells: Low speed (20-50rpm) oscillation can be used for cell scratch experiments, drug penetration tests, or to avoid excessive cell adhesion affecting experimental observation.
- Aerobic microbial culture: aerobic proliferation of bacteria such as Escherichia coli, Bacillus subtilis, lactic acid bacteria, yeast, filamentous fungi, and other fungi. Vibration can accelerate oxygen dissolution in the culture medium, meeting the respiratory needs of microorganisms.
- Research on Microbial Metabolites: Oscillatory cultivation can promote the synthesis and release of microbial metabolites (such as antibiotics, enzyme preparations, organic acids), facilitating subsequent separation and purification.
- Microbial tolerance testing: Simulate the effects of different temperatures, CO ₂ concentrations, and drug concentrations on microbial growth in an oscillating environment to evaluate their environmental adaptability or resistance.
- Recombinant protein expression: Cultivate engineering bacteria (bacteria, yeast) or engineering cells on a large scale, shake and combine with a suitable CO ₂ environment to increase the expression level of recombinant proteins (such as insulin and interferon).
- Vaccine strain cultivation: In the development of viral vaccines (such as influenza vaccines), shake culture supports the growth of viral vectors (such as adenovirus) or host cells, providing a favorable environment for virus proliferation.
- Biological product quality testing: used for sterile inspection of biological products such as vaccines and antibodies. Oscillatory cultivation can quickly detect contaminated aerobic microorganisms.
- Expansion culture of bacterial solution before plasmid extraction: Shake culture of engineered bacteria (such as DH5 α) to rapidly amplify bacteria containing the target plasmid, providing sufficient raw materials for plasmid extraction.
- Gene cloning related culture: such as preparation of competent cells, oscillation culture after screening of transformants, to ensure cell viability and proliferation efficiency, and support subsequent cloning experiments.
- Tissue engineering research: Dynamic cultivation and oscillation of tissue blocks (such as skin and cartilage tissue) can promote the infiltration of nutrients into the tissue, simulating the physiological stress state of tissues in vivo.
- Drug development and screening: Evaluate the inhibitory/promoting effects of drugs on cells and microorganisms, and use an oscillating environment to ensure full contact between drugs and culture subjects, thereby improving the accuracy of drug screening.
- Agricultural and environmental science research: such as the oscillation cultivation of plant probiotics and nitrogen fixing bacteria, or the study of the impact of environmental pollutants on microbial activity, providing data support for agricultural production and environmental governance.