Introduction to the working principle of carbon dioxide full temperature oscillation incubator
Date: 2025-12-02Read: 2
The core of the carbon dioxide full temperature oscillation incubator is achieved throughCollaborative and precise control of three major parameters: temperature, CO ₂ concentration, and oscillation stateThe stable in vitro and in vivo environment required for simulating the growth of cells and microorganisms is based on the collaborative operation of three core systems, as follows: -
Principle of precise temperature regulation
adoptCompressor cooling+electric heatingDual loop temperature control, combined with PID intelligent algorithm to achieve wide range and high-precision temperature regulation. The heating system generates heat through nickel chromium alloy heating wires, and the hot air is evenly transported to the working room through a three-dimensional air duct circulating fan to avoid local temperature differences; The refrigeration system consists of components such as compressors and condensers, which achieve low-temperature output through refrigerant circulation and meet the needs of low-temperature cultivation. The temperature sensor inside the box collects real-time temperature data and feeds it back to the control system to dynamically adjust the heating power or cooling intensity. The temperature control accuracy of conventional models can reach ± 0.1 ℃~± 0.5 ℃, with a temperature range covering -10 ℃~60 ℃, suitable for the growth temperature requirements of different biological samples. Some models are equipped with humidifiers, which adjust the humidity inside the box to 50% -95% RH through ultrasonic humidification technology to prevent cell drying and dehydration. -
Principle of Stable Control of CO ₂ Concentration
based onInfrared sensing detection+automatic air supply closed-loop controlMechanism to maintain stable concentration of CO ₂ inside the box. High purity CO ₂ is connected to the equipment through the gas cylinder interface and controlled on/off by an electromagnetic valve; The infrared sensor inside the box monitors the concentration of CO ₂ in real time. When the concentration is lower than the set value (adjustable from 0% to 20%), the control system automatically opens the solenoid valve to introduce CO ₂ into the box. At the same time, the gas mixer ensures that CO ₂ is fully mixed with the air inside the box to avoid local concentration unevenness. After the concentration meets the standard, the solenoid valve automatically closes, and the sealing structure of the box is used to reduce leakage. The infrared sensor has a fast response speed and a concentration control accuracy of ± 0.1%~± 0.3%. It also supports concentration gradient setting to meet the respiratory and metabolic needs of different cells. -
Principle of oscillation collaborative cultivation
The oscillation system is driven by a variable frequency motor to drive the eccentric wheel to move the worktable, providingReciprocating or rotary typeTwo oscillation modes. The motor can accurately adjust the oscillation frequency (0-300r/min), and the oscillation amplitude can be adjusted between 20-50mm. Through high-frequency oscillation, it accelerates the diffusion of nutrients in the culture medium, while increasing the amount of oxygen dissolved in the liquid, avoiding cell adhesion or precipitation, and promoting uniform cell growth. The workbench adopts a rigid connection design, runs smoothly, and can carry various culture containers such as triangular flasks and culture dishes, ensuring that the containers are stable during oscillation and do not affect sample growth. -
Principles of System Collaboration and Security Linkage
The three major systems are linked through a central controller to achieve synchronous parameter adjustment and real-time monitoring. The touch screen can intuitively display temperature, CO ₂ concentration, oscillation frequency, and other data, supporting program storage and automatic operation. At the same time, it is equipped with multiple safety linkage mechanisms, which automatically pause oscillation and reduce CO ₂ output when opening the door. When overheating or CO ₂ leakage occurs, an alarm is immediately triggered and relevant modules are cut off. Combined with the ultraviolet disinfection lamp inside the box, it not only ensures the safety of sample cultivation but also prevents cross contamination, providing stable guarantee for the efficient proliferation of cells and microorganisms.