Instructions for using a carbon dioxide incubator


1、 Installation and preparation
1. Location selection
Install the incubator near the water source and faucet for easy water addition and drainage. Long term placement of water receiving containers under the drainage pipes of the box to prevent water stains from polluting the environment.
2. Water source connection
Take out the water inlet hose, connect it to the water inlet on the left side of the box, and tie it tightly with a self-locking cable tie.
Connect the other end of the hose to the faucet and tighten it tightly. Turn on the faucet and fill it with water until the drain pipe comes out, indicating that it is full of water. At this time, the high water level indicator light is on, just turn off the faucet.
3. CO ₂ gas connection
Prepare pressure regulator, gas connection components, and intake hose. Insert the left screw cap of the pressure regulator into the connecting component, install it onto the regulator, and tighten it.
Connect the right connector of the regulator to the CO ₂ cylinder and tighten the screw cap clockwise with a wrench.
One end of the intake hose is inserted into the gas connection component, and the other end is inserted into the inlet of the incubator, both of which are fastened with self-locking zip ties.
4. Pressure regulation
Ensure that the pressure regulator is in the locked state and open the top switch of the CO ₂ cylinder counterclockwise.
Slowly turn the regulator switch clockwise to keep the pressure pointer on the left dial between 0.025-0.05 megapascals.
Pull out the black knob on the right side of the box and turn it clockwise to increase the intake pressure, so that the dial pointer of the box matches the dial pointer of the steel cylinder.
2、 Operation steps
1. Water injection for humidification tray
Inject half of the water into the humidification tray and place it at the bottom of the inner box to ensure normal humidity control.
2. Power supply and parameter settings
Turn on the power switch, and the control panel display screen will light up.
Temperature setting:
Press the SETT key, select the set position using the numeric shift key, adjust the value using the numeric increase/decrease key (e.g. 37 ℃), and press the confirm key to save.
CO ₂ concentration setting:
Press the CO ₂ concentration setting button, and adjust the value (such as 5%) using the digital shift button and increase/decrease button. Press the confirm button to save.
3. Startup and operation
Click the operation indicator switch, and the indicator light turns green to indicate normal operation. To stop, click the stop button.
The lighting is a delayed lighting system that automatically turns off after about 1 minute.
3、 Maintenance and Precautions
1. Regular maintenance
Filter replacement: Replace the filter every six months to ensure the effectiveness of gas filtration.
UV lamp replacement: The UV lamp needs to be replaced after 1000 hours of use, and the indicator light flashes to prompt replacement.
CO ₂ cylinder pressure: When the pressure is below 2 megapascals, replace the cylinder in a timely manner.
2. Fault handling
The door switch indicator light is on: indicating that the door is not tightly closed and needs to be closed again.
The water level indicator light on the water tray is on: it indicates that the water level is insufficient and needs to be refilled.
Over temperature protection light on: If the temperature exceeds the set value, check the heating system or ventilation situation.
4、 Safety Tips
CO ₂ cylinder pressure: Adjust the pressure slowly to avoid damaging the sensor due to excessive pressure.
High temperature sterilization: During the sterilization process, samples should not be placed inside the box to prevent sample damage.
Door lock safety: The door lock automatically starts during high-temperature sterilization to ensure safe operation.