The Soxhlet extraction method is suitable for extracting crude fat from samples with high lipid content and low bound lipid content, which can be dried and finely ground, and are not prone to moisture absorption and clumping.
Features: Simple operation, easy extraction, and easy calculation of experimental results.

Soxhlet extraction method is used for the determination of crude fat content. Fat is widely present in the seeds and fruits of many plants, and measuring the content of fat can be used as an indicator to distinguish its quality. There are many methods for determining fat content, such as extraction method, acid hydrolysis method, specific gravity method, refraction method, electrical measurement, and nuclear magnetic resonance method. At present, the extraction method is widely used both domestically and internationally, among which the Soxhlet extractor method is a classic method and also the standard method for grain and oil fractionation in China.
Experimental Principle
The residual method in the Soxhlet extraction method is adopted, which involves using low boiling point organic solvents (petroleum ether) for reflux extraction to remove crude fat from the sample. The crude fat content is calculated based on the difference in weight between the sample and the residue. Due to the presence of free fatty acids, sterols, phospholipids, waxes, pigments, and other lipid substances in organic solvent extracts in addition to fats, the results obtained by extraction method can only be crude fats.
Experimental materials
Oil crop seeds, medium speed filter paper
Instrument device
(1) Soxhlet fat extractor or JT-SXT-06 oil analyzer
(2) Drying machine (diameter 15-18cm, filled with color changing silicone gel)
(3) Stainless steel tweezers (20cm long)
(4) Culture dish
(5) Analytical balance (sensitivity 0.001g)
(6) Weighing bottle
(7) Constant temperature water bath
(8) Oven
(9) Sample sieve (60 mesh)