The specific steps for anaerobic cultivation in an anaerobic incubator are as follows:
Place necessary accessories and tools according to usage requirements.
Turn on the power, turn on the lighting, turn on the temperature controller, and adjust the desired temperature.
Place 1000g of palladium granules (sealed) and 500g of desiccant in the operating room, and put in Meilan indicator paper (sealed).
Close the indoor and outdoor doors of the sampling room tightly and perform vacuum calibration.
One replacement in the operating room (nitrogen replacement): put latex gloves on the flange ring of the observation board and tighten them, then fill with nitrogen.
Second replacement of the operating room (nitrogen replacement): Repeat the nitrogen filling process, evacuate the sampling room first, and pay attention to using the foot switch to open and close the exhaust at any time. Three consecutive nitrogen replacements.
The fourth replacement of the operating room (mixed gas replacement): Replace the gas path and open the mixed gas valve 3 for intake. When inflating, evacuate the sampling room first and use the foot switch to open and close the exhaust at any time.
Turn on the pellet deoxygenation agent in the operating room, connect the deoxygenation catalyst power for catalytic deoxygenation, and after one hour, open the Meilan indicator paper to observe its color change. If it does not change color, it indicates that the operating room has reached an anaerobic environment.
Turn on the ultraviolet sterilization lamp and sterilize indoors. The sterilization time is determined according to the specific experiment.
The above steps ensure that the anaerobic incubator can provide a strict anaerobic environment to meet the cultivation needs of anaerobic organisms.