The Hamilton diluter uses microinjection syringe technology to achieve highly accurate semi-automatic liquid absorption and discharge processes through active displacement. This technology ensures the accuracy and stability of liquid processing, providing reliable assurance for subsequent dilution operations. The diluter works based on the "proportional method", which obtains solutions of different concentrations by changing the dilution liquid volume. In practical operation, the instrument will mix high concentration samples with diluent in a specific ratio according to preset programs or user set parameters, reducing their concentration to the desired range. This instrument usually has an automatic mode, where the instrument automatically controls the entire dilution process. Users only need to adjust system parameters such as allocation ratio, volume, etc., and the instrument can automatically complete the steps of sample suction, mixing, and discharge, greatly reducing the need for manual intervention.
Precautions for using Hamilton diluter:
1. In terms of safety protection
-Wear complete personal protective equipment: Before performing any operation involving chemical reagents, operators must wear personal protective equipment such as lab coats, gloves, goggles, etc. to prevent chemical reagents from splashing onto the skin or eyes and causing injury.
-Good ventilation environment operation: Ensure that the laboratory has a good ventilation system, especially when dealing with toxic and volatile reagents, it is necessary to operate in a fume hood to avoid inhaling harmful gases.
2. Instrument maintenance and upkeep
-Regularly check the integrity and performance of components: Regularly check whether the various components of the instrument are intact and undamaged, especially whether there are any cracks or leaks in the connecting pipelines; Regularly verify the accuracy of the flowmeter and calibrate it if necessary; Clean or replace the filter to prevent particle blockage from affecting liquid flow and contaminating the sample.
-Proper cleaning of residual liquids: After each use, any residual liquids inside the instrument should be promptly cleaned. Generally speaking, it is necessary to inject water or specialized cleaning solution into the dilution and preparation instrument multiple times and discharge it to remove residual substances and avoid cross contamination between different samples. When not in use for a long time, the pipeline should be emptied and filled with protective gas.
3. In terms of operational standards
-Follow standard procedures: strictly follow the instrument manual and experimental standard operating procedures for operation, and do not arbitrarily change the order and method of operation. If encountering problems or uncertain situations, one should promptly consult with guidance personnel.
-Avoid cross contamination: Use appropriate containers and tools for dilution and distribution, and be careful to avoid cross contamination between different liquids. For example, do not use the same suction head to extract different solutions.
4. In terms of environmental factor control
-Appropriate temperature and humidity: Maintain a relatively stable temperature and humidity in the laboratory, as special temperature and humidity conditions may affect the performance of instruments and the accuracy of measurement results. The ideal laboratory environment temperature generally fluctuates less within the room temperature range, and the relative humidity is controlled within a reasonable range.
-Stay away from interference sources: Place the instrument on a stable workbench, away from vibration sources, strong magnetic fields, and other electromagnetic interference devices to avoid affecting the normal operation and measurement accuracy of the instrument.
5. Consideration of sample characteristics
-Understand the properties of the sample: Before use, fully understand the physical and chemical properties of the sample to be processed, such as acidity, oxidation-reduction, volatility, etc., in order to take corresponding protective measures and choose appropriate operating methods. For samples with special properties, additional preprocessing steps or special accessories may be required to ensure safe and effective dilution operations.