routine maintenance

Surface cleaning: Use a soft cloth dipped in a small amount of water or 70% alcohol to wipe the instrument housing every week to avoid reagent leakage and corrosion of the surface.

Sample hole maintenance: Clean the sample hole dust with an ear wash ball or soft bristled brush every 3 months to prevent contamination from affecting testing; If contaminated with fluorescent substances, soak in 90% alcohol for 20 minutes and let it air dry naturally.

Hot cover maintenance: Regularly wipe the hot cover with alcohol or pure water to ensure good temperature control performance; After the experiment, maintain standby for 10 minutes until the module temperature drops to room temperature before turning off the power.

Environmental requirements: The instrument should be placed in a dry (humidity<85%) environment without strong magnetic field interference, with a temperature controlled between 18-35 ℃ and a space of 20-30cm around to ensure heat dissipation.

calibration method

Temperature calibration: Place a precision temperature probe (accuracy ≥ 0.01 ℃) in the heating module, collect data through a temperature calibration device, adjust the instrument temperature to the set value, and ensure the accuracy of the temperature control system.

Fluorescence calibration: Introduce a DNA template with known fluorescence intensity as a standard substance, detect the fluorescence signal and construct a standard curve to verify the linear relationship between the signal and concentration.

CT value calibration: By amplifying a known concentration template, calculate the linear relationship between CT value and template concentration to ensure amplification efficiency between 90% -110%.

Common troubleshooting

Abnormal fluorescence signal: Check if the fluorescent probe or dye has expired, clean the fluorescent filter and laser surface to ensure the quality of the reaction system is qualified.

Unstable temperature control: Check if the heating module and cooling system are working properly, recalibrate the temperature sensor to avoid environmental temperature fluctuations affecting the results.

Software or connection issues: Update or reinstall the software, check if the USB cable is loose, and ensure a stable connection between the computer and the instrument.

Low amplification efficiency: Optimize template concentration and reaction system, redesign primers to ensure no contamination of reagents and templates.