Daily maintenance and upkeep methods for 48 position positive pressure solid-phase extraction instrument
Date: 2025-11-25Read: 0
As the core equipment for batch sample pretreatment in the laboratory, the stability of the 48 bit positive pressure solid-phase extraction instrument directly affects the accuracy and repeatability of the detection data. Daily maintenance should revolve around the core of "smooth pipeline, stable pressure, and clean components", combined with the characteristics of multi-channel parallel work. The specific methods are as follows:
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Cleaning of pipelines and channelsAfter the experiment, rinse the channel continuously with organic solvents such as methanol and acetonitrile for 3-5 minutes, and then rinse with ultrapure water for 2-3 minutes to avoid residual crystallization of the sample blocking the pipeline; Disassemble the injection needle and extraction column connector, wipe off residual contaminants with a cotton swab, and ensure good sealing of the interface.
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Pressure system inspectionAfter turning off the gas source, observe whether the pressure gauge remains stable (pressure drop ≤ 0.02MPa/30 minutes). If there is a leak, apply soapy water to the pipeline interface and valve for troubleshooting; Clean the filter screen of the pressure regulating valve to avoid impurities affecting the accuracy of pressure control.
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Sample tray and pallet cleaningRemove the sample tube holder and collection bottle tray, rinse with warm water and air dry. Stubborn stains can be wiped with alcohol to prevent cross contamination; Check if the positioning holes on the tray are deformed to ensure that the sample tubes and collection bottles are securely placed.
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Valve group and sealing ring maintenanceDisassemble the six way valve and solenoid valve, clean the valve core with anhydrous ethanol ultrasonic cleaning for 5-10 minutes, air dry and reinstall; Check the sealing rings of each interface. If aging or deformation occurs, replace them immediately. During installation, apply a small amount of silicone grease to enhance the sealing performance.
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Maintenance of positive pressure pumpClean the intake filter of the pump body to avoid dust blocking and affecting the intake efficiency; Check the pump oil level (oil bath type), replenish specialized pump oil if insufficient, and replace it promptly if the oil quality is turbid to ensure smooth operation of the pump body.
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Instrument appearance and environmental organizationWipe the instrument casing with a damp cloth to remove dust and residual reagents; Keep the laboratory ventilated and dry to avoid circuit failures caused by humid environments. Do not stack debris around the instruments.
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Comprehensive maintenance of pipelinesDismantle all connecting pipelines, soak them in organic solvents for 30 minutes, rinse them, check whether the pipelines are aging or cracking, and replace damaged pipelines in a timely manner; Calibrate the flow sensor to ensure that the flow deviation of each channel is ≤ 5%.
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Cleaning of Extraction Column Adaptation ComponentsClean the fixed clamp and positioning seat of the extraction column, remove residual column packing and sample impurities, check the elasticity of the clamp, and ensure that there is no air leakage after the installation of the extraction column.
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Circuit and control system inspectionOpen the side cover of the instrument and clean the dust on the circuit board with the cold air mode of the hair dryer; Check if the power and data cables are securely connected to prevent poor contact from causing the instrument to crash or parameter drift.
- After the experiment, thoroughly rinse the pipelines and channels with methanol to remove residual solvents and prevent pipeline corrosion.
- Turn off the gas source, release the pressure inside the pipeline, remove vulnerable components (such as sealing rings and injection needles), clean and seal for storage.
- Cut off the power, cover the instrument with a dust cover, and store it in a dry, cool environment, avoiding direct sunlight and high temperature and humidity.
- During maintenance, it is necessary to turn off the power and gas sources, and it is strictly prohibited to operate with electricity or pressure to avoid the risk of pipeline rupture or electric shock.
- Choose organic solvents compatible with the instrument (such as methanol, acetonitrile) to avoid damaging pipelines and valve groups with highly corrosive reagents.
- Replace parts with original factory accessories to ensure model matching and avoid affecting instrument performance due to improper accessories; Regularly record maintenance logs to facilitate tracing the cause of faults.