IF=26.8 | Jiete Biological Cell Embedding Dish Helps with High Quality Research

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Date: 2025-10-28Read: 2

Jiete Biotechnology

The first stock of consumables listed on the Science and Technology Innovation Board of China's biological laboratory

Dedicated to providing comprehensive laboratory solutions for researchers

We have over 12000 products and accessories, including:

Laboratory consumables, process consumables

Biological reagents, laboratory instruments, medical devices

Our product quality has remained consistent for 24 years

To ensure that researchers receive

Best repeatability and reliable results

Exported to over 70 countries and regions

Always widely used

To better support the development of scientific research work

Since 2014 until now

Over the past 10 years

We have been consistently conducting SCI paper collection activities

Jiete Biotechnology - Cell Embedding Dish

Assist in the publication of high-quality papers

Recently, the team of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, published a high-quality paper in the magazine Advanced Materials A Universal Strategy for BBB Transport Mediated by an Inflammatory Receptor Antagonist for Neuroprotection in Ischemic Stroke， Jiete Biotechnology's cell embedding dish was used in its research to construct a blood-brain barrier model.

article interpretation

01 Research Results

A universal strategy for blood-brain barrier pathway mediated by inflammatory receptor antagonists: neuroprotection for ischemic stroke

In the treatment of ischemic stroke, the selective penetration of therapeutic drugs through the blood-brain barrier (BBB) is one of the main obstacles. After cerebral ischemic injury, the expression of various inflammatory receptors on brain microvascular endothelial cells is upregulated, providing potential targets for receptor-mediated endocytosis.

This study proposes a universal drug delivery strategy: using inflammation receptor antagonists (such as Serat, SQ29548, S18886, etc.) as targeted ligands, co assembling with oligomerized anthocyanin-3-glucoside (C3G) to form nanoparticles (CCAs). These antagonists can help the nanoparticles cross the BBB and regulate the inflammatory response, thereby achieving neuroprotection.

Schematic diagram of antagonist coupled nanoparticles using receptor targeting strategy for the treatment of ischemic stroke

02 Research Methods

In vitro experiments using cell embedding dishes

In the in vitro experiments of this study, a blood-brain barrier model (upper cerebral endothelial cells, lower cerebral nerve cells) was constructed using Jiete Biotechnology's cell embedding dish, and the ability and mechanism of action of C3G based nanoparticles combined with inflammatory receptor antagonists to cross the blood-brain barrier model and achieve neuroprotection were verified.

Schematic diagram of BBB model constructed by cell embedding dish (left)

The Jiete Biological 24 well cell embedding dish used in this study (right)

Verification 1

Evaluate the ability of a series of C3G based nanoparticles that bind to corresponding antagonists or agonists to cross the blood-brain barrier, receptor-mediated effects, as well as anti-inflammatory and antioxidant effects using a cell embedded dish BBB model. (including nanoparticles CC lacking receptor targeting ligands; antagonist modified nanoparticles CCS, CCSQ, CCS18, CCBD, CCNC; Excitant modified nanoparticles CCU4, CC1V)

A) Through Cy3 fluorescence monitoring, different formulations were internalized in PC12 cells in the lower chamber, indicating successful penetration of the blood-brain barrier. In addition, the antagonist group showed significantly stronger fluorescence signals, indicating that the presence of antagonists promoted receptor-mediated cross cellular transport;

B) By Cy3 fluorescence monitoring, the bEnd. 3 cells in the upper chamber uptake CC, CCS, CCSQ, CCS18, and CCU4. Evaluate the competitive inhibition of nanoparticle uptake by co incubation with corresponding free antagonist ligands. The uptake of antagonist modified nanoparticles by endothelial cells significantly increased, confirming effective receptor-mediated endocytosis;

The relative expression levels of tumor necrosis factor alpha and arginin-1 in PC12 cells treated with C-F free ligands or different nano formulations. The antagonist group significantly inhibited the expression of pro-inflammatory cytokine TFN - α and upregulated the expression of anti-inflammatory cytokine Arg-1, indicating that they have immunomodulatory and anti-inflammatory effects while targeting;

G) The ROS levels in PC12 cells after OGD and subsequent CCA treatment were evaluated by DCFH-DA staining. The ROS related fluorescence in the antagonist group was significantly reduced, indicating their strong antioxidant activity.

Verification 2

The transport pathway and protective effect of inflammation receptor antagonist modified nanoparticles represented by CCS on endothelial cells were studied using a BBB model embedded in cells, as well as the restoration of blood-brain barrier integrity and its permeability after therapeutic intervention.

A) Schematic diagram of BBB model constructed using cell embedding dish; Schematic diagram of CCS transport pathways within cells (lysosome escape, Golgi mediated exocytosis, and blood-brain barrier recovery response);

B) Immunofluorescence staining of Tx2AR inflammatory receptor on bEnd. 3 cell membrane and its coexistence with CC and CCS. CCS exhibits higher signal intensity, indicating an antagonist mediated mechanism that supports nanoparticle delivery by improving cross cell interactions across the blood-brain barrier;

C-E confocal imaging shows the coexistence of CCS with lysosomes, Golgi apparatus, and mitochondria in endothelial cells. Verified the dual transport pathway of CCS: a portion of CCS NPs escape the lysosomal compartment for endothelial structure repair, while the other portion is delivered to neurons through the Golgi apparatus to exert antioxidant and anti-inflammatory effects;

F) Quantitative analysis of experimental results in Figures C, D, and E;

G) Transendothelial resistance (TEER) measurement reflecting the integrity of bEnd. 3 monolayer barrier. The CCS group showed significant reparative effects;

H) Scratch healing model image of BECs. The endothelial repair performance of the CCS group is superior to all other groups.

Disclaimer: The images accompanying the tweet are sourced from a journal, and our company does not assume any legal liability for infringement

Jiete Biotechnology - Cell Embedding Dish

Accurate simulation empowers scientific research

01 Product Introduction

Cell embedding dishes are commonly used in various cell experiments to simulate the primitive growth environment of cells using membrane technology, making cells grown in vitro more similar in morphology and function to those grown in vivo.

Jiete Biotechnology's cell embedding dish is made of polycarbonate (PC) and polyethylene terephthalate (PET) transparent films, which have high pore density and can complete various transmembrane material exchanges. They are widely used in cell co culture, cell molecule transport and other experiments for studying cell functions such as transport, absorption and secretion. This product offers a variety of membrane pore sizes and specifications to choose from, as well as insertable culture dishes to meet customers' diverse experimental needs.