use LUNA-III ™ Automatic cell countingceremonyPerform reliable and reproducible cell counting

Keywords: inter device and intra device deviation, consistency, repeatability, reliability, automatic cell counter

1 Introduction

Accurate cell counting is crucial for maintaining experimental integrity in biotechnology research. cellcountThe differencepossiblyWill lead to significant experimental resultsdifferenceEspecially in the fields of cell therapy production, quality control, and biomanufacturing, precise cell quantification plays a decisive role in ensuring product quality and compliance with regulatory standards.Dian ChengLUNA-III ™ The automatic cell counter provides researchers with an economical and efficient solution to achieve accurate and reliable cell counting. This study evaluatesIt's doneLUNA-III ™ Performance performance, focusanalyzeIts ability to maintain consistency of results between a single device and multiple devices,Emphasize the importance of reducing differences in the experimental process.

2 Materials and Methods

All cell counts were performed usingDian ChengLUNA-III ™ Default detection program. To evaluate the equipmentinsideDeviation,useSantaiDian ChengLUNA-III ™ According to the standardQuality Control ProcedurePerform seven repeated counts on standard microspheres. equipmentofintervalofDeviation is achieved through five different machinesDian ChengLUNA-III ™ Verify. alltestAll use loadingAt DianchengLUNA ™ Standard microspheres on cell counting slides (item number B13101).

2.1 Point completionLUNA-III ™ Within and between devicesofDeviation assessment

The experiment employed3T3 cell line was cultured in DMEM medium containing 10% fetal bovine serum and 1% bispecific antibody. Cell viability testing was performed using 0.4% trypan blue staining solution (item number T13101).In addition,Use six typesNISTTraceable particle size standard（9.98, 20.85, 30.04, 43.33, 50.00, and 57.43um）comeVerify particle size measurementofAccuracy.

2.2 NIST cantrace backParticle size standardAccuracy of

as shown in the tableAs shown in Figure 1, all test results maintain good consistency, with highly consistent parameters such as total cell concentration, average cell size, and total cell number. Minor changes (CV value of approximately 1%) may be caused by re inserting LUNA cell counting slides during repeated cell counting using the device.

table1. Dian ChengLUNA-III ™ Deviation analysis within the equipment. Three different devices were used to test three batches of standard microspheres,

Each device performs five repeated counts on each batch of samples.

Reproducibility between devices through five unitsDian ChengLUNA-III ™ Evaluate the testing of standard microspheres from the same batch. As shown in Table 2, there are differences between devicesveryLow, allmeasurementCoefficient of variation of parameters（The CV remains below 2%, fully demonstrating the high consistency between different devices and ensuring reliable results regardless of which device is used.

table2. Dian ChengLUNA-III ™ Deviation analysis between devices. Using five different LUNA-III ™ The equipment tests the same batch of standard microspheres and repeats the experiment twice.

3 Conclusion

This study confirms thatDian ChengLUNA-III ™ Automatic cell counter in equipment andBetween devicesAccurate and reproducible cell counting results can be provided in all tests. Coefficient of variation of all detection parameters（The continuous decrease in CV below 2% fully demonstrates the high reliability of the device in measuring cell concentration and size. theseThe results confirm了Dian ChengLUNA-III ™ asaA trustworthy cell quantification tool in the field of scientific research that can provide researchers with accurate cell quantification results.