Case Study | Turbidity Meter Used for Determination of Antibacterial Activity of Lactic Acid Bacteria

densitometers-in-the-determination-of-antibacterial-activity-of-lactic-acid-bacteria

Overview of Lactic Acid Bacteria

self1929Since British scientist Fleming discovered penicillin in, antibiotics have made significant contributions to the advancement of human medicine. However, the widespread and sustained use of antibiotics in clinical practice has led to the development of resistance in pathogenic microorganisms. At present, the resistance of pathogenic microorganisms to antibiotics is threatening people's health, and the search for alternative compounds with antibacterial activity has become a focus of scientific attention.

Lactic acid bacteria have entered people's vision. This type of bacteria is widely present in the human gut and can utilize fermentable carbohydrates to produce large amounts of lactic acid, which is of great significance for maintaining gut health. Lactic acid bacteria and their metabolites are also widely used in the pharmaceutical and food industries as probiotics and natural preservatives.

Most importantly, it can produce small molecules that can inhibit the growth of bacteria, fungi, yeast, and even eukaryotic cells——Bacteriocins (bacteria)iocins)Therefore, it has antibacterial activity. Bacteriocins have stable properties and low toxicity, and are known asThe new generation of anti infection warriors. Lactic acid bacteria with antibacterial activity provide a direction for solving the problem of drug resistance.

Determination of Antibacterial Activity of Lactic Acid Bacteria

Among lactic acid bacteria, Lactobacillus plantarum(L.plantarum)yescommonOne of the bacterial strains that can produce various antibacterial components such as organic acids, hydrogen peroxide, diacetyl, bacteriocins, and antimicrobial peptides with different activity spectra. In medicine, Lactobacillus plantarum, as a probiotic, has been proved to play an active role in the treatment of intestinal diseases, metabolic syndrome, diabetes, obesity, etc.

2019In [year], researchers from the Russian Federation Ministry of Health's Ogamalei Epidemiology and Microbiology Research Center utilized point formationGrant's Den-1The turbidity meter was used to study the antibacterial effect of plant lactobacillus supernatant on Gram positive and Gram negative pathogens. In order to investigate the antibacterial activity of plant derived lactobacilli against Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes, and Staphylococcus aureus, the researchers first usedDEN-1Prepare bacterial suspension using turbidity analyzer, add the supernatant of Lactobacillus plantarum and incubate18h， And measure 540 nmThe optical density at the site is used to determine the bacterial growth rate. The results indicate that plant lactobacilli have inhibitory effects on the growth of the tested bacteria.

Determination of antibacterial activity of point forming organismsSolution

As a laboratory instrument integrator, Dian Cheng BiologyAlways committed to providing customers with advanced laboratory equipment solutions. Dian ChengGrantDEN-1Turbidity meter is a compact instrument for measuring the turbidity of bacterial suspensions, which can meet the needs of various life science applications.DEN-1The measurement range is0.00~15.00McFarland， The measurement time is as short as 1 second. If you point to Grant DEN-1If you are interested in turbidity analyzer products, please feel free to contact us at any time.