Fully automatic nucleic acid extractorIt plays a crucial role in the process of nucleic acid extraction, but may also encounter some malfunctions and common problems. Here are some common troubleshooting and solutions:

1、 The instrument cannot be started

Possible reasons: power supply issues, blown fuses, or internal circuit faults.

resolvent:

Check if the power socket is supplying power normally.

Check if the fuse on the back of the instrument is blown. If it is blown, replace it with a new fuse.

If both the power supply and fuse are normal, it may be an internal circuit fault and the manufacturer needs to be contacted for repair.

2、 No display or abnormal display on the screen

Possible reasons: Display screen issues or loose connection wires.

resolvent:

Check if the display screen connection cable is loose. If it is loose, unplug and plug the connection cable again.

If the connection cable is normal, it may be a display screen malfunction and a new display screen needs to be replaced.

3、 The operation interface is unresponsive or slow to respond

Possible reasons: Touch screen malfunction or internal software issues.

resolvent:

Try restarting the instrument to see if it can solve the problem.

If the problem persists after restarting, it may be a touch screen malfunction and a new touch screen needs to be replaced.

If the touch screen is functioning properly, it may be an internal software issue and the manufacturer needs to be contacted for software upgrade or repair.

4、 Low extraction efficiency

Possible reasons: Inappropriate ratio of magnetic beads to samples, quality issues with magnetic beads, malfunction of magnetic bead separator, improper sample and reagent ratio, insufficient grinding, insufficient eluent, or improper operation.

resolvent:

Check if the ratio of magnetic beads to samples is appropriate, and adjust the ratio according to the instructions.

Check the quality of magnetic beads and replace them with new ones if necessary.

If the ratio of magnetic beads to samples and the quality of magnetic beads are normal, it may be a malfunction of the magnetic bead separator and the manufacturer needs to be contacted for repair.

Ensure that the sample size and reagent ratio are controlled within a certain range to avoid exceeding the lysis capacity.

Grind the sample thoroughly to ensure that the nucleic acid extraction conditions are met.

Wash with sufficient eluent and mix thoroughly to avoid any residual lumps.

5、 Leakage occurred during the extraction process

Possible reasons: worn sealing ring, blocked pipeline, or pump malfunction.

resolvent:

Check if the sealing ring is worn. If it is worn, replace it with a new one.

If the sealing ring is normal, check if the pipeline is blocked. If there is any blockage, clean the pipeline.

If the sealing ring and pipeline are both normal, it may be a pump failure and the manufacturer needs to be contacted for repair.

6、 The extraction result is unstable

Possible reasons: reagent quality issues, inaccurate temperature control, improper operation, or mismatched experimental consumables.

resolvent:

Check the quality of the reagents and replace them with new ones if necessary.

Check if the temperature control system is accurate, and make adjustments if there are any deviations.

Strictly follow the instructions to avoid unstable results caused by improper operation.

Ensure that the experimental consumables are matched, such as using appropriate adsorption columns or magnetic beads.

7、 Excessive noise

Possible reasons: Fan malfunction, motor malfunction, or loose internal components.

resolvent:

Check if the fan is running properly, and replace it with a new one if there are any abnormalities.

If the fan is functioning properly, check if the motor is running smoothly. If there are any abnormalities, contact the manufacturer for repair.

If both the fan and motor are normal, it may be due to loose internal parts, and the manufacturer needs to be contacted for inspection and repair.

In addition, there are some specific onesThe fully automatic nucleic acid extractorCommon problems and their solutions:

8、 There is a significant difference in the results between manual extraction and machine extraction

Possible reasons: There are differences between the instrument operation process and the manual operation process, such as affecting the efficiency of the combination of cracking and binding steps.

Solution: Optimize the system of machine operation to ensure the efficiency of steps such as cracking, binding, washing, and elution.

9、 There are differences in extraction efficiency between instrument holes

Possible reasons: The hole position does not fit tightly with the bottom heating groove or there is a problem with the instrument heating module.

Solution: Check the fit between the hole position of the deep hole plate and the bottom heating groove to ensure a tight fit. At the same time, observe whether there are residual magnetic beads in the upstream cleavage well of the well position, and check whether the transfer magnetic bead ability of the instrument between the well positions is complete. If the problem persists, consider whether there is a problem with the instrument heating module and contact the manufacturer for repair.

10、 There is a significant difference between the volume of eluent added during machine extraction and the actual volume of nucleic acid solution obtained

Possible reason: Improper design or operation of the elution well may result in evaporation or residue of the elution solution.

Solution: Ensure the correct operation of the elution step and avoid evaporation or residue of the elution solution. At the same time, check whether the design of the elution hole is reasonable, and if necessary, contact the manufacturer for consultation or adjustment.

The fully automatic nucleic acid extractor may encounter various malfunctions and common problems during use. In order to ensure the normal operation of the instrument and the accuracy of the extraction results, users should regularly maintain and upkeep the instrument, and strictly follow the instructions for operation. When encountering problems, promptly contact the manufacturer or professional maintenance personnel for resolution.