50% yolk solution is used forAnaerobic yolk agar basic medium（027165）Supporting reagents

Anaerobic Egg Yolk Agar Base

【Anaerobic yolk agar baseUsage】

Used for the inspection and cultivation of Clostridium botulinum in imported and exported food (SN/T 0865-2000)

【 Principle of Inspection 】

Yeast powder, tryptone, and peptone provide nitrogen sources, vitamins, and growth factors; Glucose provides a carbon source; Sodium chloride is used to maintain cell osmotic pressure; Agar is a coagulant.

【Anaerobic yolk agar basic mediumFormula]

Formula (per liter) content

Yeast extract 5.0g

Tryptone 5.0g

Monthly peptone 20.0g

Sodium chloride 5.0g

Agar 15.0g

Distilled water 1000mL

Final pH 7.0 ± 0.2

【 Usage 】

1. Weigh 50 g of anaerobic yolk agar base medium, add 1000 mL of distilled water or deionized water, stir and heat to boil until dissolved, and sterilize at 121 ℃ for 15 minutes. Cool to around 50 ℃, add 10-15 mL of 50% sterile yolk saline suspension to every 100ml of culture medium, shake well, and immediately pour onto a plate.

2. Take an appropriate amount of fungal moss with an inoculation ring, gradually dilute it with sterile 0.85% physiological saline at a ratio of 1:100 or 1:100 to the appropriate concentration, inoculate by pouring, and incubate in a 35 ℃ -37 ℃ incubator for 24 hours.

3. Result observation attachment: 50% yolk solution: Take fresh eggs, wash the eggshells with a hard brush, drain them, soak them in a 70% alcohol solution for 1 hour, remove the yolk with sterile operation, add an equal amount of sterile physiological saline, mix well and set aside