The goal of recombinant protein production is to produce high-yield functional proteins. This application guide describes the production process of recombinant human phosphoserine phosphatase (hPSP) for conceptual system validation. These cultures can be harvested using CR30NX or CR22N high-speed ground centrifuge paired with R9A2 fixed angle centrifuge (4x1.5L triangular centrifuge bottle). This product combination is efficient and flexible, laying the foundation for large-scale production workflows of recombinant proteins.