The determination method of the second-hand Thermo Fisher ICS-600+ion chromatograph includes steps such as startup preparation, sample processing, instrument operation, data analysis, and shutdown maintenance. It is necessary to strictly follow the specifications to ensure accurate and reliable results. The following is its detailed measurement method:
1、 Preparation before startup
Check the status of the instrument: Ensure that the instrument is in good condition, without damage or abnormal conditions.
Preparation of reagents: Prepare corresponding standard solutions, eluents, samples, etc. according to the analysis needs. The preparation of eluent should be carried out according to the instructions of the chromatographic column or the requirements of different standards. For example, for anion determination, carbonate system can be used, and for cation determination, methanesulfonic acid system can be used.
Connecting pipelines: Ensure that the injection pipeline, eluent pipeline, etc. of the ion chromatograph are connected correctly and without leakage.
Water supply and exhaust: Place the filter head in the deaerated ultra pure water, turn on the power and deaeration switch for online deaeration, adjust the flow rate to an appropriate value, turn on the constant flow pump switch, and flush the flow path to remove bubbles.
2、 Startup and initialization
Turn on the power: sequentially turn on the power switches of the instrument, computer, column constant temperature host, etc., and the instrument enters the preheating stage.
Initialization settings: According to the instrument prompts, perform necessary initialization operations, such as setting time, date, etc.
Establish analysis methods: Establish corresponding analysis methods on the instrument software, including parameter settings such as eluent concentration, flow rate, and injection volume.
3、 Sample analysis
Sample preparation: The sample to be analyzed is subjected to necessary pretreatment, such as filtration, dilution, etc., to ensure that the sample meets the analysis requirements.
Injection: Inject the processed sample into the injector to ensure accurate injection volume. For an automatic sampler, it is necessary to correctly place the sample bottle and set the injection sequence according to the operating instructions.
Start analysis: Start the analysis program on the instrument software, and the instrument will automatically perform sample analysis. During the analysis process, the instrument will display real-time chromatograms, and users can observe the progress of the analysis.
4、 Data analysis and storage
View chromatogram: After the analysis is completed, the instrument will generate the corresponding chromatogram. Users can view chromatograms and analyze ion components in samples through software.
Data processing: Based on the chromatogram, users can calculate the concentrations of various ions in the sample and perform necessary data processing, such as standard curve fitting, concentration calculation, etc.
Save data: Save the analysis results as a data file for future reference and sharing.
5、 Shutdown and maintenance
Cleaning the pipeline: After the analysis is completed, clean the pipeline with the corresponding cleaning solution to ensure that there is no residue inside the pipeline.
Shutdown: Follow the instrument prompts to correctly turn off the power and related equipment of the ion chromatograph.
Maintenance: Regularly maintain the instrument, such as replacing the shower solution, cleaning the pump head, checking and replacing the online filter head, to ensure long-term stable operation of the instrument.
6、 Precautions
Safe operation: When operating the chromatograph, it is necessary to follow the safety operating procedures to avoid accidents.
Sample pretreatment: Sample pretreatment is crucial for the analysis results. Users should ensure that the sample pretreatment process is correct and error free to reduce analysis errors.
Regular calibration: Regularly calibrate the instrument to ensure the accuracy of the analysis results.
Avoid cross contamination: When replacing samples or rinsing solutions, it is necessary to clean the tubing and injector to avoid cross contamination.