Common configurations:

• CO ₂ concentration: 1-5% (commonly used for Chlorella vulgaris, 2-5%)

• Temperature: 20-30 ℃ (Chlamydomonas reinhardtii 25 ℃, diatoms 20 ℃)

• Light intensity: needs to be combined with an external light source (50-200 μ mol photons/m ²/s)

• Shake speed: 100-150 rpm (promotes uniform distribution of CO ₂ and nutrients)

Principle:

• Typical scenario: Cultivation research of microalgae biodiesel raw materials (such as Chlorella vulgaris and Scenedesmus)

• CO ₂ concentration1-5% (also adjusted according to the type of algae, such as 2-5% commonly used by Chlorella vulgaris).
  Principle: Algae, like plants, can carry out photosynthesis, and CO ₂ is one of the raw materials for photosynthesis. Different algal species have different requirements for CO ₂. Adding more CO ₂ to Chlorella can make its photosynthesis more vigorous and grow faster, laying the foundation for subsequent oil production.

• Temperature requirements20-30 ℃ (different algae species are also subdivided, for example, Chlamydomonas reinhardtii is suitable for 25 ℃, diatoms are suitable for 20 ℃).
  Principle: Temperature can affect the enzyme activity inside algal cells. Enzymes are the "catalysts" for cellular metabolism. If the temperature is not suitable and the enzyme activity is low, the growth and metabolism of algae will slow down; Only by selecting the right temperature and high enzyme activity can algae efficiently divide and accumulate oil.

• illuminanceIt is necessary to combine external light sources and control the intensity at 50-200 μ mol photons/m ²/s.
  Principle: Algae convert light energy into chemical energy (stored, including energy storage substances such as oils and fats) through photosynthesis. The light is too weak, the photosynthesis is insufficient, and the energy is not enough; Excessive light exposure may damage the photosynthetic structure within algal cells. So we need to choose a suitable range in the middle, so that the algae can "eat enough but not hold on".

• Shaker speed100-150 rpm (to promote uniform distribution of CO ₂ and nutrients).
  Principle: In the liquid used to cultivate algae, CO ₂ tends to float upwards