In the daily experimental process, ifIncubator repeatedappearPollution needs to be combinedThe core of the two-step operation of "daily preventive disinfection" and "post pollution cleaning" is to destroy the microbial breeding environment. The specific methods are as follows:

1、 Daily preventive disinfection (to avoid repeated pollution)

1. Regularly clean the interior: every1-2Empty the incubator on a weekly basis and use75%Medical alcohol wipes are used to wipe the inner walls, partitions, and trays (removable parts can be soaked in alcohol)5Focus on cleaning corners, door gaps, and other areas that are prone to dust accumulation.

2.UV disinfection: After each use or at the end of each day's experiment, turn on the UV lamp inside the box (ensure there are no samples) and irradiate it30-60Minutes, disinfect and ventilate10Add the sample in minutes (ultraviolet radiation can produce ozone, so avoid direct inhalation).

3. Humidity water tray management: The water tray needs to be filled with sterile or distilled water and replaced weekly1Next, when changing the water, wipe the inner wall of the water tray with alcohol to prevent bacteria from breeding in the water.

4. Sample standard placement: Sample bottle/The dish should be sealed properly to avoid direct opening; Separate the placement of different types of samples to reduce the risk of cross contamination.

2、 Disinfection after pollution (removing existing pollution)

1. Emptying and preliminary cleaning: Take out all samples (contaminated samples need to be sterilized separately), wipe the inside with alcohol wipes, and then use0.1%Chlorine containing disinfectant (such as84Disinfectant, according to1:500Dilute and wipe the inner wall, then let it stand20After minutes, wipe off any residue with sterile water (to avoid corroding metal parts).

2. High temperature dry heat sterilization: If the incubator supports the "dry heat sterilization mode" (generally the highest temperature)160-180℃), can be set160℃ sterilization2Hour (requires removal of non high temperature resistant components such as rubber seals); If this mode is not available, "low-temperature formaldehyde fumigation" can be used instead (professional operation is required to avoid formaldehyde residue).

3. Individual sterilization of components: detachable parts such as partitions, trays, water trays, etc. can be placed in high-pressure steam sterilization pots（121℃、103kPa，20Kill stubborn microorganisms such as spores in minutes.

4. Recovery and monitoring: Ventilation after disinfection24Hour, put into sterile culture medium blank control（37℃ cultivation24-48After confirming the absence of bacterial growth, use it normally.

Key precautions

-Avoid using high concentration alcohol (such as95%）Or strong acid and strong alkali disinfectants to prevent corrosion of the inner chamber and sensors of the incubator.

-If contamination occurs repeatedly, it is necessary to check whether the seal of the incubator door is aging (loose sealing can leak bacteria), or whether the sample itself carries bacteria (the sterility of the sample needs to be confirmed before inoculation).