Preclinical animal ultrasound microbubble contrast agentIt is a micrometer sized bubble wrapped in inert gases (such as perfluoropropane and sulfur hexafluoride) using liposomes or polymer materials as the shell, with a diameter usually ranging from 1-10 microns, and can freely pass through capillaries. The core principle is to generate nonlinear signals (such as harmonics) through the vibration of microbubbles under ultrasound, significantly enhancing the contrast between tissue and blood vessels, thereby improving the clarity and resolution of ultrasound images

　　Preclinical animal ultrasound microbubble contrast agentApplication fields:

Cardiovascular disease diagnosis:

Myocardial perfusion assessment: auxiliary observation of ventricular wall motion, diagnosis of atrial or ventricular diaphragmatic defects, assessment and location of myocardial ischemia, auxiliary diagnosis of coronary heart disease, balloon dilation evaluation, and postoperative vascular re occlusion tracking.

Angiogenesis analysis: Abnormal blood vessels in malignant tumors exhibit a "fast in, fast out" pattern, such as a significant delay in the peak time of contrast agents in canine lung adenocarcinoma.

Tumor diagnosis and treatment:

Tumor angiogenesis analysis: Enhance vascular imaging of the tumor site through ultrasound contrast agents to improve the sensitivity of tumor detection.

Biopsy and ablation guidance: Accurately locate the active and necrotic areas of the tumor, guide the puncture path, and reduce false negative rates.

Drug loaded microbubble therapy: Utilizing the cavitation effect of drug loaded microbubbles mediated by ultrasound to achieve targeted drug release and achieve the efficacy of local chemotherapy for tumors.

Abdominal organ evaluation:

Evaluation of splenic lesions: Benign lesions such as nodular hyperplasia and hematoma show equal or uniform enhancement; Malignant lesions such as angiosarcoma and lymphoma are characterized by late low enhancement, rapid regression, and twisted blood supply vessels.

Evaluation of liver and pancreatic diseases: CEUS can distinguish between metastatic tumors (uneven enhancement) and benign nodules (uniform filling in the portal vein phase) in distinguishing liver nodules; In terms of pancreatitis assessment, contrast-enhanced imaging showed increased blood flow, which differed significantly from normal pancreatic tissue.

Gastrointestinal and urinary system imaging:

Gastrointestinal water imaging: Injecting physiological saline containing microbubbles orally or by enema to dilate the lumen and clearly display the mucosal and muscular layers.

Urography: evaluation of urethral stricture, injection of shaking saline or SonoVue ®， Real time observation of urethral patency; The detection of bladder rupture and the strong echo signal of microbubble extravasation into the abdominal cavity have higher sensitivity than conventional ultrasound.