Program cooling device is a device that achieves controllable cooling rate through pre-set programs, widely used in fields such as cell cryopreservation, biological sample preservation, and material performance testing. Its core value lies in reducing sample damage caused by sudden temperature changes through refined temperature regulation. The following is a detailed explanation of the standard usage process:
1、 Preliminary preparation
1. Equipment inspection
-Confirm that the power connection is stable and there are no alarm prompts on the display screen; Check if the internal chamber is clean and dry. If there is any residual moisture, wipe it with a dust-free cloth.
-Select appropriate loading containers (such as cryovials, culture plates) according to experimental requirements, ensuring their size is compatible with the instrument.
2. Reagent and sample pretreatment
-Prepare a protective agent with a gradient concentration (such as DMSO cell cryopreservation solution), and add it to the sample to be frozen in proportion and mix thoroughly.
-Pack the processed samples into dedicated containers, label them with numbers, and match them with experimental records.
3. Parameter pre programming
-Open the control system and enter the program editing interface. Set the cooling curve based on the sample type: initial temperature → phased cooling nodes → final target temperature.
*Example scheme *: Room temperature (25 ℃) → Reduce to 4 ℃ at a rate of -1 ℃/min, maintain for 30 minutes, and then continue to reduce to -80 ℃ at a rate of -5 ℃/min.
-Set up automatic save program and conduct simulation testing to verify the feasibility of the program.
2、 Operation implementation
1. Sample loading
-Open the insulated door, place the container containing the sample steadily on the tray support, close the door and lock the seal tightly.
-Ensure that the sensor probe is close to the sample area to avoid temperature deviation caused by contact with metal parts.
2. System startup
-Select the stored program files, double check the parameters for accuracy, and then start the cooling program.
-At the initial stage, closely observe the real-time temperature curve and compare it with the preset value to determine if there is any abnormal deviation. If the deviation exceeds ± 2 ℃, immediately pause the program to investigate the cause (such as sensor detachment, environmental interference).
3. Process monitoring
-Use supporting software to collect real-time temperature time data and generate dynamic curve graphs. Focus on the temperature plateau phenomenon during the phase transition stage (such as the solution freezing period).
-Record key parameters every 15 minutes, including actual temperature, remaining time, and equipment operating status.
3、 Termination and aftermath
1. End of program processing
-When the temperature reaches the target value and stabilizes for 10 minutes, the system automatically stops cooling. At this point, slowly open the hatch to retrieve the sample.
-If long-term storage is required, quickly transfer to a liquid nitrogen tank or ultra-low temperature refrigerator to avoid heating and thawing.
2. Data archiving
-Export a complete temperature record report, labeled with sample number and operator information. Analyze whether the cooling curve meets expectations and evaluate the effectiveness of the protective agent.
3. Equipment maintenance
-Clean the condensed water inside the cavity and disinfect the inner wall by wiping it with 75% alcohol. Check the pressure gauge of the refrigeration system and add environmentally friendly refrigerant if necessary.
-Perform a monthly no-load calibration to verify temperature control accuracy.
4、 Precautions
-Safety protection: Wear anti freezing gloves during operation to avoid direct contact with ultra-low temperature components; It is strictly prohibited to forcefully open or close the cabin door while the equipment is running.
-Emergency response: In the event of a power outage, immediately activate the backup power supply to maintain a low temperature state; If there is a leakage, quickly cut off the power and contact the engineer for maintenance.
-Personalized optimization: Based on different sample characteristics such as volume and thermal conductivity, it is necessary to adjust the cooling rate through multiple experiments and establish a dedicated protocol template.