Product Introduction

This kit is used for quantitative analysis of mouse culture cell supernatant and mouse serum samplesIL-2TheIL-4TheIL-6TheIL-10TheIL-17ATheIFN-γTheTNF-αThe concentration. This reagent kit is based on flow cytometry analysis, and the detection principle is shown in the diagram1As shown, in7Attach corresponding mouse cytokine antibodies to specific fluorescent encoded microspheres. When specific antigens are detected in the sample, these capture antibodies linked to fluorescent encoded microspheres will bind to them, and finally, phycoerythrin will be added（PE）Labeling specific detection antibodies to form microsphere linked capture antibodies-antigen-PEThe labeled detection antibody complex. Phycocyanin in flow cytometry（APC）Under the channel,7Cluster microspheres are recognized and used to determine antigens; In flow cytometryPEUnder the channel, on the microspherePEThe fluorescence intensity is positively correlated with the concentration of each detected substance in the sample, and the corresponding fluorescence signals of each calibration standard can be fitted into a concentrationThe fluorescence intensity standard curve can be used to calculate the concentration of each antigen through the curve equation.

picture1Schematic diagram of high-throughput detection of cytokines using flow cytometry fluorescence method6Taking cytokine detection as an example

Fluorescent encoded microspheres and corresponding detection substances