Synthecon was founded in 1990 by inventors from NASA's Cell Research Program The Rotary Cell Culture System (RCCS) has been redesigned with NASA's technology transfer and is suitable for basic medicine, drug development, and other clinical research
Traditional staticcell cultureIt is carried out in a culture bottle or dish Both cells and tissues grow in a two-dimensional plane and come into contact with glass or plastic surfaces This approach will affect the expression of genes in cells and prevent sustained growth and differentiation At the same time, cells cultured in a flat culture also undergo dedifferentiation, causing the cultured cells to gradually lose many physiological characteristics of their source tissue In most dynamic culture systems, cells or tissues are suspended by physical external forces, and many factors, including liquid shear forces, can cause damage to cells and tissues
RCCS is a disruptive three-dimensional microgravity cultivation system It uses a culture disk or culture column for cultivation, adding culture medium, cells or tissues together into the culture disk or culture column, and removing all bubbles The culture disk or culture column is installed on a base with a rotating motor, and the internal tissues, cells, or cell clusters remain suspended due to the dual effects of rotational tangent force and gravity As cells or tissues grow, the rotational speed can be adjusted. After the cells form clusters, the rotational speed must be increased to prevent them from settling and touching the bottom The purpose of rotation is to allow all cells to exchange nutrients and gases evenly, and there should be sufficient contact between cells, which is conducive to cell aggregation In addition, both the culture disk and the back side of the culture column are equipped with silica gel gas exchange membranes to facilitate gas exchange, allowing cells/tissues to obtain sufficient oxygen and eliminate metabolic waste gases
The cells or tissues grown in it are suspended in a free fall state without destructive pressures such as stirrers or bubbles. Therefore, the tissues can freely fall, flip, and fully mix with the culture medium. The forces in all directions inside the container are balanced, so the cells/tissues will not be affected by a single direction of force and can grow uniformly in any direction. It is the most effective system on the market that can promote the free growth and differentiation of cells, increase cell proliferation rate, reduce cell death, and effectively increase cell product secretion Moreover, compared to other 3D cell culture systems, Syntheson's RCCS system can overcome the limitations of endogenous growth that have long plagued 3D cell culture, making it truly suitable for cultivating engineered tissues for use in drugs, medical research, regenerative medicine, and cell therapy

RCCS brings cell research into more diverse and advanced fields, with a wide range of applications that have significant value in both academic and clinical research Whether it is cultivating human tissues for research on therapeutic drugs, or cultivating alternative tissues for regenerative medicine and cell therapy, such as liver, skin, and bone marrowCartilage, myocardium, lungs, or other tissues all provide * research systems to achieve conditions that are closest to the in vivo environment Scientists can also use this system to treat tumorsCells, virusesOr other products that can produce proteins, enzymes, hormones, antigens, or antibodiesThe cultivation of cells containing important substances RCCS systems also provide ex vivo culture environments, which researchers can use to explore the effects of microenvironmental factors on cell differentiation and function

Liver tissue cultured with liver cells in Syntheson 3D system

Successfully cultured lung tissue using lung cells in the Syntheson 3D system