Microscopic fluorescence spectroscopy is a technique that combines microscopy and fluorescence spectroscopy analysis to accurately detect the luminescent properties of materials at the micrometer scale. It locates small areas through a microscopic optical system, excites samples with specific wavelengths of light, and synchronously collects their emitted spectra and fluorescence lifetime data. An analytical method used to study the optical properties and spectral characteristics of substances at the microscale. The core principle is to utilize fluorescence phenomenon, that is, certain molecules (fluorescent groups) emit longer wavelengths of light after absorbing specific wavelengths of light. By measuring and analyzing these fluorescence signals, the material composition, structure, and dynamic information of the sample can be obtained.
The core of this technology lies in its high resolution, with a spatial resolution of up to 2 micrometers and a temporal resolution of 1 nanosecond, covering the wavelength range of 200-900 nanometers. It is widely used in fields such as semiconductor materials, nanoparticles, and bioluminescence labeling, for studying micro luminescence mechanisms, defect analysis, and component identification.
Technical Principle
Fluorescence phenomenon: Fluorescent groups absorb excitation light and transition to a high energy level, then return to a low energy level and release fluorescence with a longer wavelength than the excitation light. This process follows Stokes' law, which states that the wavelength of the emitted light is greater than the wavelength of the excitation light.
Spectral analysis: Fluorescence signals are dispersed into spectra by wavelength using a spectrometer, and the fluorescence intensity of each wavelength is detected to form a characteristic spectral curve. The spectral peak positions, shapes, and intensities of different fluorescent groups have * properties, which can be used for substance identification and quantitative analysis.
Microscopic imaging: Combining microscope technology, the excitation light is focused onto a small area (such as a single cell or nanomaterial) to achieve high spatial resolution fluorescence signal acquisition, while obtaining the morphology and spectral information of the sample.
maintenance
Daily cleaning: Wipe the outer shell with a clean damp cloth and avoid using organic solvents such as alcohol. Regularly inspect dust prevention facilities and maintain cleanliness around the instrument.
Light source maintenance: Xenon lamps should not be frequently turned on and off. After turning off, they need to be cooled for half an hour before restarting to extend their lifespan. When reaching the service life (usually 500 hours), it should be replaced in a timely manner.
Environmental requirements: The instrument should be placed in a dry, vibration free, and horizontal position, with at least 0.3m of space reserved around it for heat dissipation. The power supply needs to be stable, and it is recommended to equip it with a voltage regulator.
Long term storage: If not in use for a long time, turn on the device 1-2 times a week for about half an hour each time. When storing, charge the battery to 50% and avoid humid environments.