Microscopic Raman spectrometer is an instrument that combines Raman spectroscopy analysis technology with microscopic analysis technology, used for molecular structure analysis of sample micro regions. Microscopic Raman spectrometer is mainly used for micro area chemical composition analysis, which can achieve non-destructive detection and micro level spatial resolution for substance composition identification.
The core principle of a micro Raman spectrometer is the Raman scattering effect. When a monochromatic laser (such as 532nm, 632.8nm, or 785nm) irradiates the sample, most of the light undergoes Rayleigh scattering (frequency remains constant), while a small amount of light undergoes frequency changes due to molecular vibration or rotational energy level transitions, forming Raman scattering light. This frequency change is directly related to the molecular structure of the substance, and each substance has a Raman spectral "fingerprint". By designing a confocal optical path, the instrument focuses the laser beam onto the micrometer level area (such as 1-2 μ m spot), combined with a high-resolution microscope and CCD detector, it can accurately capture scattered light signals and analyze them into spectral data.
Main functions
Micro area analysis: The excitation light spot can be focused to the micrometer level to accurately analyze the micro area of the sample. Through CCD identification instrument and TV monitoring instrument, the laser action site can be clearly displayed, and the interested sample site can be selected for analysis.
Molecular structure identification: Based on the characteristic Raman spectra of different substances, it can reflect the differences in chemical composition and molecular structure of the sample at the molecular level, thereby qualitatively identifying the substances in the sample.
Key Operating Points
Environmental control: Operate in a shockproof and constant temperature (20 ± 5 ℃) environment to avoid vibration and temperature fluctuations that may affect data accuracy.
Regular calibration: Use standard light sources (such as mercury lamps) to calibrate wave number accuracy, resolution, and reproducibility, ensuring stable instrument performance.
Sample processing: Select a suitable wavelength laser based on the properties of the sample to avoid fluorescence interference; The thickness of liquid samples needs to be controlled to prevent scattered light attenuation.
Safety protection: Laser may cause harm to the eyes, and protective goggles should be worn during operation to avoid direct eye contact with the light path.