In daily liquid chromatography（In HPLC analysis, the chromatographic column is the most valuable "consumable" in the laboratory. The correct cleaning and storage methods can not only prolong the service life of chromatography columns, but also help us avoid many common analytical problems. Today, let's learn about chromatography columns togetherMaintenance pointsGo ahead!

1Why clean the chromatography column?
1. To avoid a decrease in column efficiency: buffer solutionion pairing agentAcid and other components are prone to remain in the column, and if not cleaned, it will accelerate the aging of the column.

2. Prevent analysis issues such as increased pressure, abnormal peak shape, and the appearance of ghost peaks, which are often related to the columnRelated to the deposition of internal impurities.

Therefore, when using mobile phases that may cause column degradation, cleaning must be performed!

IICleaning methods for daily use

1. Wash with a mobile phase of the same composition and free of components that may cause deterioration (buffer solution, ion pair reagent, acid, etc.) for 15 to 30 minutes.

2. If stored for a long time, please replace it with the mobile phase used at the factory.

For example, usingUnder the condition of methanol/20 mmol/L phosphate buffer=20/80, the following operation can be performed:

1）useWash with methanol/water=20/80 for about 15 minutes.

2）If you continue to use it within a week, seal and store it.

3. If not used for more than a week, it needs to be cut offchangeTo the factorytimeThe preservation solution (methanol)/Water=70/30, then seal and store.

This ensures that the column remains stable in its next use.

IIICleaning methods when problems occur

Dissolve any adsorbed impurities or precipitated samples/Wash with salt solvent for 30 to 60 minutes. Do not use alkaline solutions or strong acids as they may damage the chromatography column.

1Adsorption of hydrophobic compounds：

Wash with methanol first 30 pointsbellIf there is no improvement, thenattemptStronger solubilityagent(such as THF、chlorineImitation).

2Salt precipitation occurs:

use firstpureRinse with water 30 pointsbellThen use methanol again/15 points for water system cleaningbell.

Caution: Avoid using strong acid or alkaline solutions to prevent color damagescoreColumn.

4Storage method of chromatographic column

1Short term custody: Using analysistimeThe flowmoveJust seal it together.

2longPeriodic storage: ConstructionDiscussion on ReplacementAs a factory storage solution (such as ACN/water=70/30) before sealing and storing.

closekeyPoint:

1. Storing under conditions of high organic solvent concentration can effectively prevent the growth of microorganisms.

2. Before each shutdown, check if cleaning is necessary to avoid column damage caused by residual buffer solution or acidic conditions.

5summary

1. ColorscoreColumns are consumables, butProper cleaning and storage can significantly delaylonglifespan

2. When using buffer solution or acidic mobile phase, it is necessary to perform appropriate cleaning.

3. When problems arise, choose a suitable solvent for targeted treatment.

4longPreservation periodtime，pleaseUse the recommended factory storage solution.

letIplurality marker (们)Develop good pillars togetherMaintain habits，letevery timeHPLCMore analysis is neededsmoothEfficient!