In the microscopic world of life code, a precision device called the Six One Agarose Horizontal Electrophoresis Instrument is helping researchers interpret the molecular information of DNA and RNA with its stable and reliable separation ability. As one of the basic equipment in molecular biology laboratories, agarose gel electrophoresis is responsible for the important task of separating and identifying nucleic acid molecules.

01 Working principle: Molecular dance in electric field

The core principle of agarose electrophoresis is based on the synergistic effect of charge effect and molecular sieve effect. Nucleic acid molecules (DNA/RNA) carry a negative charge in solutions above their isoelectric point, and when placed in an electric field, these negatively charged molecules move towards the anode.

The three-dimensional network structure formed by agarose gel acts as a "molecular sieve". Under the action of electric field, the migration rates of nucleic acid molecules of different sizes in the pores of gel are different: smaller molecules move faster, and larger molecules move slower.

This separation effect enables researchers to judge the molecular size of nucleic acid fragments according to their position on the gel, and determine the molecular weight of the sample to be tested by comparing it with known standards. The migration speed is inversely proportional to the relative molecular weight.

02 Instrument Design:

The Six One Agarose Horizontal Electrophoresis Instrument incorporates multiple practical innovations in its design. Its bridge design effectively saves buffer solution usage while ensuring uniform electric field distribution. The transparent top cover with perforated design is convenient for heat dissipation and real-time observation of electrophoresis during the experimental process.

The electrode system is the core component of the electrophoresis instrument. The Liuyi product uses platinum electrode wires with a purity of ≥ 99.95% to ensure excellent conductivity and durability. The electrode holder is designed to be detachable for easy cleaning and maintenance, extending the service life of the equipment.

In terms of safety performance, the June 1st electrophoresis instrument is equipped with high flexibility wires and a cover opening power-off safety design to ensure safe operation. The gel tray is equipped with a fluorescent scale for easy observation and recording of results. The shell is made of polycarbonate injection molding, which is resistant to high temperatures and does not deform, with no risk of leakage.

03 Product Series and Technical Parameters

Provide multiple models of agarose gel electrophoresis apparatus to meet different experimental needs. The overall dimension of DYCP-31CN is 260 × 116 × 76mm, the specification of gel plate is 100 × 70mm or 50 × 70mm, and the total capacity of buffer solution is about 260ml.

DYCP-31DN has more functions and can produce four gel of different sizes: 120 × 120mm (large gel), 120 × 60mm (wide gel), 60 × 120mm (long gel) and 60 × 60mm (small gel). This multi size selection allows it to adapt to various sample loading requirements.

DYCP-32A is a medium size electrophoresis instrument, with an overall dimension of 300 × 162 × 110mm, a gel plate specification of 160 × 100mm, and a total buffer capacity of 650ml. This model uses platinum electrode and symmetrical electrode layout, with a fixed electrode spacing of 10cm, which can output 0-150V stable DC voltage, and the uniformity error of electric field strength is ≤ 5% to ensure clear separation of strips.

04 Operation process: From glue making to imaging

The operation process of agarose horizontal electrophoresis apparatus starts from the preparation of gel. Firstly, weigh an appropriate amount of agarose powder and add it to electrophoresis buffer (usually TAE or TBE). Heat and dissolve it, then cool it to 50-60 ℃. Pour it into the gel making mold and insert a comb.

After the gel is solidified, the comb is pulled out to form a sampling hole. When pulling out the comb, pay attention to gently tilting it to pull out one side first, and then removing it as a whole to avoid damaging the sample hole. Put gel into electrophoresis tank, add buffer solution to immerse gel, and the liquid level should be 1-2mm higher than the surface of gel.

Next is sample preparation and sample addition. After mixing the nucleic acid sample with the loading buffer, add it to the sample well using a micropipette. Glycerol or sucrose in the loading buffer increases the sample density, causing the sample to sink to the bottom of the loading well; Tracer dyes such as bromophenol blue are used to indicate the progress of electrophoresis.

The electrophoresis parameter settings need to be adjusted according to experimental requirements. Generally, the voltage is set at 5-10V/cm (gel length), and the electrophoresis time is 30-120 minutes. When connecting the power supply, pay attention to the polarity of the electrodes: connect the sample hole end to the negative electrode and the other end to the positive electrode.

Finally, there is observation and analysis of the results. After electrophoresis, the gel needs to be dyed with nucleic acid dye (such as EB or SYBR Green), and then observed and photographed under the UV gel imaging system. By comparing with DNA/RNA markers, the size and content of sample nucleic acid fragments can be determined.

05 Application scenario: Basic tools for multiple fields

In genomics research, the 61 agarose gel electrophoresis apparatus is used for the separation and identification of DNA fragments, revealing the structure and function of the genome. The field of clinical diagnosis also cannot do without this tool. By performing agarose electrophoresis on samples such as serum and urine, disease markers can be quickly and accurately detected, improving the early diagnosis rate of diseases. In gene cloning and vector construction experiments, models such as DYCP-32A can verify enzyme cleavage efficiency and product integrity, ensuring the success rate of subsequent ligation and transformation experiments, and are the "gatekeepers" of gene cloning experiments. In the field of teaching, the ease of operation and stability of results of the June 1st electrophoresis instrument make it an ideal choice for molecular biology experimental courses in universities, helping students master the principles and experimental techniques of electrophoresis.